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      Differential Effects of Fibronectin-Derived Oligopeptides on the Attachment of Rabbit Lens Epithelial Cells in vitro

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          Abstract

          Among the Arg-Gly-Asp (RGD)-containing sequences that are known to be cell-binding domains of fibronectin, 500 µg/ml of Arg-Gly-Asp-Ser (RGDS) and Gly-Arg-Gly-Asp-Ser showed 100% inhibition of the attachment of cultured lens epithelial cells (TOTL-86 cells), when they were added to culture medium and coincubated with the cells for 24 h whereas RGD at concentrations of 500, 1,000 and 2,000 µg/ml had no such activity. After 48 h of cocultivation of 800, 400 or 200 µg/ml of RGDS with TOTL-86 cells, the percentage of floating cells was 100, 30.1 or 11.1%, respectively. After 144 h of cocultivation with RGDS, the percentage of floating cells was 1.6,2.4 or 1.9%, respectively, indicating that RGDS was not cytotoxic to lens epithelial cells. However, replacing the medium with fresh medium containing new RGDS peptide resulted in floating of cells. We also studied the inhibitory effect of two other amino acid sequences that are found in cell-binding sites of the fibronectin molecule: Glu-Ile-Leu-Asp-Val-Pro-Ser-Thr (EILDVPST) and Arg-Glu-Asp-Val (REDV). At 500 and 1,000 µg/ml, respectively, neither EILDVPST nor REDV has an inhibitory effect on the attachment of TOTL-86 cells, while RGDS at a concentration of 500 µg/ml completely inhibited the attachment of the cells in 24 h of incubation.

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          Author and article information

          Journal
          ORE
          Ophthalmic Res
          10.1159/issn.0030-3747
          Ophthalmic Research
          S. Karger AG
          0030-3747
          1423-0259
          1996
          1996
          11 December 2009
          : 28
          : 4
          : 201-208
          Affiliations
          aDepartment of Ophthalmology, Osaka Prefectural Habikino Hospital, Habikino-shi, bDepartment of Ophthalmology, Osaka University Medical School, Suita-shi, cDepartment of Industrial Chemistry, Wakayama National College of Technology, Gobou-shi, dDepartment of Ophthalmology, Osaka National Hospital, Osaka-shi, and eLaboratory of Biochemistry, Osaka Prefectural College of Nursing, Habikino-shi, Japan
          Article
          267904 Ophthalmic Res 1996;28:201–208
          10.1159/000267904
          8878182
          4a8364d8-d023-4843-9969-4af0c099301a
          © 1996 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          History
          : 21 April 1995
          : 28 November 1995
          Page count
          Pages: 8
          Categories
          Original Paper

          Vision sciences,Ophthalmology & Optometry,Pathology
          After-cataract,RGDS peptide,Lens epithelial cells,Fibronectin,Attachment

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