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      Cellular antioxidant activity (CAA) assay for assessing antioxidants, foods, and dietary supplements.

      1 ,
      Journal of agricultural and food chemistry
      American Chemical Society (ACS)

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          Abstract

          A cellular antioxidant activity (CAA) assay for quantifying the antioxidant activity of phytochemicals, food extracts, and dietary supplements has been developed. Dichlorofluorescin is a probe that is trapped within cells and is easily oxidized to fluorescent dichlorofluorescein (DCF). The method measures the ability of compounds to prevent the formation of DCF by 2,2'-azobis(2-amidinopropane) dihydrochloride (ABAP)-generated peroxyl radicals in human hepatocarcinoma HepG2 cells. The decrease in cellular fluorescence when compared to the control cells indicates the antioxidant capacity of the compounds. The antioxidant activities of selected phytochemicals and fruit extracts were evaluated using the CAA assay, and the results were expressed in micromoles of quercetin equivalents per 100 micromol of phytochemical or micromoles of quercetin equivalents per 100 g of fresh fruit. Quercetin had the highest CAA value, followed by kaempferol, epigallocatechin gallate (EGCG), myricetin, and luteolin among the pure compounds tested. Among the selected fruits tested, blueberry had the highest CAA value, followed by cranberry > apple = red grape > green grape. The CAA assay is a more biologically relevant method than the popular chemistry antioxidant activity assays because it accounts for some aspects of uptake, metabolism, and location of antioxidant compounds within cells.

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          Author and article information

          Journal
          J Agric Food Chem
          Journal of agricultural and food chemistry
          American Chemical Society (ACS)
          0021-8561
          0021-8561
          Oct 31 2007
          : 55
          : 22
          Affiliations
          [1 ] Department of Food Science, Cornell University, Ithaca, New York 14853-7201, USA.
          Article
          10.1021/jf0715166
          17902627
          4aaf717f-71ad-4e95-9150-c3729b7b5d67
          History

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