Field ecologists often rely on mark-release-recapture (MRR) experiments to estimate population dynamics parameters for a given species. In the case of a medically important taxon, i.e., a disease vector, inferences on species survival and dispersal rates are particularly important as they have the potential to provide insights into disease transmission dynamics in endemic areas. Medical entomologists have traditionally used fluorescent dusts to externally mark the cuticle of insects. However, dust marking is usually restricted to the adult life stage because immature insects lose the mark when they molt.
We evaluated the efficacy of 13 trace elements in marking nymphs of three native Brazilian Chagas disease vectors: Triatoma brasiliensis, Triatoma pseudomaculata, and Rhodnius nasutus. Cr and Cu were detected in over 97% of T. brasiliensis (34/35 31/31 for Cr and Cu), while Cu and Mn were detected in more than 95% of T. pseudomaculata (29/29 for Cu and 28/29 for Mn) tested 120 days after marking. Only Mn marked over 90% of R. nasutus nymphs (38/41). Overall, trace elements had no negative effects on T. pseudomaculata longevity, but As-marked T. brasiliensis nymphs (p<0.01), and Cd-marked R. nasutus nymphs (p<0.01) had significantly shorter lifespan.
Previous evidence shows that there is little or no genetic differentiation between populations at the microgeographic level, which often precludes indirect estimations of dispersal capability based on genetic markers. In such situations, MRR studies are more suitable as they measure insect movement directly from one site to another, instead of effective migration (i.e. gene flow). The determination of a reliable and persistent marking method is the first step towards the development of meaningful ecological estimates through the application of MRR methodology. Here, we have identified trace elements that can be used for mark and recapture studies of three triatomine species in Brazil.
Mark, release and recapture (MRR) is one of the most powerful techniques employed by field ecologists, and is used to provide relevant estimates of natural populations such as dispersal, survival rates, and population densities. A reliable, persistent and effective marking method is a sine qua non condition to conduct a MRR experiment. Despite their relevance as Chagas disease vectors in the Americas, triatomine bugs have seldom been subjected to MRR studies. So far, estimates on triatomine dispersal have largely relied on molecular/genetic approaches, which are not direct measurements of the distance between the release and recapture point. We evaluated the persistence and efficiency of trace elements, chemicals present in very low quantities in the organism of a targeted species, to mark the nymphs of three Chagas disease vectors of the Brazilian caatinga biome, Triatoma brasiliensis, T. pseudomaculata, and Rhodnius nasutus. At least one trace element was shown to be a good marker for each triatomine species: more than 97% of T. brasiliensis were still marked with Cr and Cu after 120 days; more than 95% of T. pseudomaculata were marked with Cu and Mn and more than 90% of R. nasutus were marked with Mn 120 days after marking. Only As-marked T. brasiliensis and Cd-marked R. nasutus nymphs had significantly shorter lifespan. We believe these findings show that trace elements are a reliable and persistent marking method for triatomine nymphs and hope this is the first step towards the development of meaningful ecological estimates through the application of the MRR methodology.