A list of spider species recorded from the Parque Estadual Mata São Francisco, Paraná, Brazil was compiled based on 7,942 specimens, of which 2,872 are adults (36.15%) and 5,071 are juveniles (63.85%). Adults were identified as belonging to 45 families, 140 genera and 209 species and morphospecies (101 nominal species and 108 morphotypes). Forty-one species were recorded for the first time from the state of Paraná, most of them belonging to Araneidae (14), Oonopidae (4), Theridiidae (4), and Uloboridae (3). Conifaber guarani Grismado, 2004 and Oonops nigromaculatus Mello- Leitão, 1944 were recorded for the first time from Brazil. These results place Paraná as the sixth state with the highest number of records of spiders from Brazil, currently 465 species. This study increases in 10% the number of species recorded from Paraná, and the Atlantic Forest fragment becomes one of the most well sampled areas in the state, with 20% of all known species in Paraná.