Feature Selection for the Automated Detection of Metaphase Chromosomes: Performance Comparison Using a Receiver Operating Characteristic Method

Abstract

Background. The purpose of this study is to identify a set of features for optimizing the performance of metaphase chromosome detection under high throughput scanning microscopy. In the development of computer-aided detection (CAD) scheme, feature selection is critically important, as it directly determines the accuracy of the scheme. Although many features have been examined previously, selecting optimal features is often application oriented. Methods. In this experiment, 200 bone marrow cells were first acquired by a high throughput scanning microscope. Then 9 different features were applied individually to group captured images into the clinically analyzable and unanalyzable classes. The performance of these different methods was assessed by a receiving operating characteristic (ROC) method. Results. The results show that using the number of labeled regions on each acquired image is suitable for the first on-line CAD scheme. For the second off-line CAD scheme, it would be suggested to combine four feature extraction methods including the number of labeled regions, average regions area, average region pixel value, and the standard deviation of either region distance or circularity. Conclusion. This study demonstrates an effective method of feature selection and comparison to facilitate the optimization of the CAD schemes for high throughput scanning microscope in the future.