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      Dissecting microRNA-mediated gene regulation and function in T-cell development.

      1 ,
      Methods in enzymology
      Elsevier BV

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          Abstract

          MicroRNAs (miRNAs) are abundant approximately 22-nucleotide regulatory RNAs encoded in animal genomes. They are thought to exhibit diverse biological functions in animals by targeting messenger RNAs (mRNAs) for degradation or translational repression. Here we use T-cell development as a model to illustrate methods and strategies for dissecting the post transcriptional gene regulatory networks controlled by miRNAs and their roles in the differentiation of T-cell precursors. The process involves the identification of miRNA genes in rare T-cell progenitors, determining miRNA expression during T-cell development, characterizing miRNA function in T-cell development using an in vitro assay, and identifying functionally relevant gene(s) regulated by miRNAs.

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          Author and article information

          Journal
          Meth. Enzymol.
          Methods in enzymology
          Elsevier BV
          0076-6879
          0076-6879
          2007
          : 427
          Affiliations
          [1 ] Department of Microbiology and Immunology, Baxter Laboratory of Genetic Pharmacology, Stanford University School of Medicine, Stanford, California, USA.
          Article
          S0076-6879(07)27010-7
          10.1016/S0076-6879(07)27010-7
          17720485
          4b708ad4-7c7f-471b-9493-d56ebbe7486b
          History

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