In this research, dispersion of a new type of one-dimensional inorganic material Nb 2Se 9, composed of van der Waals bonds, in aqueous solution for bio-application study were studied. To disperse Nb 2Se 9, which exhibits hydrophobic properties in water, experiments were carried out using a block copolymer (poloxamer) as a dispersant. It was confirmed that PPO, the hydrophobic portion of Poloxamer, was adsorbed onto the surface of Nb 2Se 9, and PEO, the hydrophilic portion, induced steric hinderance to disperse Nb 2Se 9 to a size of 10 nm or less. To confirm the adaptability of muscle cells C2C12 to the dispersed Nb 2Se 9 using poloxamer 188 as dispersant, a MTT assay and a live/dead assay were performed, demonstrating improvement in the viability and proliferation of C2C12 cells.