Mest (also known as Peg1), an imprinted gene expressed only from the paternal allele
during development, was disrupted by gene targeting in embryonic stem (ES) cells.
The targeted mutation is imprinted and reversibly silenced by passage through the
female germ line. Paternal transmission activates the targeted allele and causes embryonic
growth retardation associated with reduced postnatal survival rates in mutant progeny.
More significantly, Mest-deficient females show abnormal maternal behaviour and impaired
placentophagia, a distinctive mammalian behaviour. Our results provide evidence for
the involvement of an imprinted gene in the control of adult behaviour.