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      Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects.

      Nature medicine

      Weight Loss, Adult, Animals, Body Mass Index, Energy Intake, European Continental Ancestry Group, Fasting, Female, Humans, Immunoblotting, Indians, North American, Leptin, Male, Mice, Mice, Inbred Strains, Mice, Obese, Middle Aged, Obesity, blood, ethnology, Proteins, analysis, genetics, RNA, metabolism, Rats, Rats, Mutant Strains

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          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Leptin, the gene product of the obese gene, may play an important role in regulating body weight by signalling the size of the adipose tissue mass. Plasma leptin was found to be highly correlated with body mass index (BMI) in rodents and in 87 lean and obese humans. In humans, there was variability in plasma leptin at each BMI suggesting that there are differences in its secretion rate from fat. Weight loss due to food restriction was associated with a decrease in plasma leptin in samples from mice and obese humans.

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          Most cited references 39

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          Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.

          A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of undegraded RNA in high yield and can be completed within 4 h. It is particularly useful for processing large numbers of samples and for isolation of RNA from minute quantities of cells or tissue samples.
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            Positional cloning of the mouse obese gene and its human homologue.

            The mechanisms that balance food intake and energy expenditure determine who will be obese and who will be lean. One of the molecules that regulates energy balance in the mouse is the obese (ob) gene. Mutation of ob results in profound obesity and type II diabetes as part of a syndrome that resembles morbid obesity in humans. The ob gene product may function as part of a signalling pathway from adipose tissue that acts to regulate the size of the body fat depot.
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              A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity

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                Author and article information

                Journal
                7584987

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