+1 Recommend
1 collections
      • Record: found
      • Abstract: found
      • Article: found

      Delayed-Type Hypersensitivity Skin Reaction in the Chronically Uremic Mouse: Influence of Severity and Duration of Uremia on the Development of Response


      S. Karger AG

      Experimental renal failure, Chronic renal failure, Chronic Uremia, Secondary Immunosuppression

      Read this article at

          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.


          A technique of delayed-type hypersensitivity (DTH) skin reaction to a contact sensitizing agent, oxazolone, was used to assess the effects of uremia on cell-mediated immunity in chronically uremic mice and, for comparison, sham-operated and normal controls. The first objective was to establish that DTH responses are reduced in animals with renal failure. To do this, mice were made uremic by a combination of electrocoagulation of the entire surface of one kidney and subsequent contralateral nephrectomy; studies included biochemical and hematological evaluation using blood urea nitrogen level and hemoglobin concentration as routine indices to assess the degree and consequence of uremia, respectively. The second objective was to apply the technique to observe and compare changes during uremic states of varying severity and duration. A modest, although significant, decrease in both the induction and maintenance of DTH responses was observed in the mice with severe renal failure only (BUN above l00mg/dl). This immunosuppressive effect was manifest early and persisted unchanged throughout the entire observation period (3–9 weeks). This study presents new evidence that severe uremia readily produces in the mouse sustained, albeit mild, changes in cell-mediated immunity. Furthermore the ability to elicit DTH skin reaction in the chronically uremic mouse offers a versatile system for studying changes in cell-mediated immunity occurring during uremia with a broad range of potential applicability.

          Related collections

          Author and article information

          S. Karger AG
          05 December 2008
          : 43
          : 1
          : 16-21
          Renal Division, Department of Medicine, Montreal General Hospital, McGill University, Montreal, Que., Canada
          183711 Nephron 1986;43:16–21
          © 1986 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 6
          Original Paper


          Comment on this article