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      • Record: found
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      • Article: found

      Light and Electron Microscopic Studies of the Rat Hyalocyte after Perfusion Fixation

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          Abstract

          The characteristic morphology of a not intentionally stimulated hyalocyte is described, using TEM and perfusion fixation. The best results were obtained by retrograde perfusion via the abdominal aorta with a glutaraldehyde- and formaldehyde-containing fixative. The cells, situated in the cortical area of the vitreous body, show mostly an indented nucleus, primary and secondary lysosomes, mitochondria, cisterna, clusters of free ribosomes and a Golgi apparatus. Bristle-coated micropinocytotic vesicles can also be distinguished and in some cells a centriole is visible. Sometimes the cells show many cytoplasmic protuberances. Morphologically the majority of those cells can be considered as resting macrophages.

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          Author and article information

          Journal
          Journal ID (publisher-id): ORE
          Journal ID (nlm-ta): Ophthalmic Res
          Journal ID (doi): 10.1159/issn.0030-3747
          Title: Ophthalmic Research
          Publisher: S. Karger AG
          ISSN (Print): 0030-3747
          ISSN (Electronic): 1423-0259
          Publication date Subscription-year: 1985
          Publication date (Print): 1985
          Publication date (Electronic): 04 December 2009
          Volume: 17
          Issue: 3
          Pages: 125-130
          Affiliations
          aDepartment of Ophthalmology, bLaboratory for Cell Biology and Histology, and cHaematology and Immunology Unit, Free University of Brussels (VUB), Belgium
          Article
          Publisher ID: 265363 Other ID: Ophthalmic Res 1985;17:125–130
          DOI: 10.1159/000265363
          PubMed ID: 4011125
          SO-VID: 4c9e7bb0-4b9a-40d6-b9d5-4992830e6ede
          Copyright © © 1985 S. Karger AG, Basel
          License:

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          History
          Date received : 17 February 1984
          Date accepted : 05 June 1984
          Page count
          Pages: 6
          Categories
          Subject: Original Paper

          ScienceOpen disciplines: Vision sciences,Ophthalmology & Optometry,Pathology
          Keywords: Vitreous body,Hyalocytes,Perfusion fixation,Light and electron microscopy,Rat
          Data availability:
          ScienceOpen disciplines: Vision sciences, Ophthalmology & Optometry, Pathology
          Keywords: Vitreous body, Hyalocytes, Perfusion fixation, Light and electron microscopy, Rat

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