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      Overexpression of lncRNA GAS5 suppresses prostatic epithelial cell proliferation by regulating COX-2 in chronic non-bacterial prostatitis

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          ABSTRACT

          Chronic non-bacterial prostatitis (CNP) is a common urologic disease that is linked to the development of prostate cancer. Long non-coding RNA (lncRNA) GAS5 has been identified to mediate cell proliferation in prostate cancer, although its role in CNP is still unclear. Human prostate epithelial cell line RWPE-1 was induced by lipopolysaccharide (LPS) to mimic CNP model in vitro. Real-time PCR was performed to determine the expression of GAS5 and COX-2, while western blotting was used to evaluate the protein expression of COX-2. The interaction between GAS5 and COX-2 was determined using RNA pull-down and RNA immunoprecipitation (RIP). Cell proliferation was determined using MTT assay. The expression of GAS5 was decreased, while COX-2 was increased in prostatitis tissues and in LPS-induced RWPE-1 cells. The overexpression of GAS5 suppressed the protein level of COX-2, and inhibited cell proliferation of LPS-induced RWPE-1 cells and HPECs, which was rescued by the co-transfection with pcDNA-GAS5 and pcDNA-COX-2. GAS5 was confirmed to promote the ubiquitination of COX-2, and the in vivo GAS5-overexpressed CNP rat model decreased the motor scores, the volume of prostate tissues, the average number of inflammatory cells, prostatic proliferation, and COX-2 expression. Our findings revealed that overexpression of GAS5 inhibited cell proliferation via negatively regulating the expression of COX-2, thus alleviating the progression of CNP.

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          Author and article information

          Journal
          Cell Cycle
          Cell Cycle
          KCCY
          kccy20
          Cell Cycle
          Taylor & Francis
          1538-4101
          1551-4005
          2019
          21 April 2019
          : 18
          : 9
          : 923-931
          Affiliations
          [a ] Department of Urology, The First Affiliated Hospital of Soochow University , Suzhou, Jiangsu, China
          [b ] Reproductive Medicine Center, The First Affiliated Hospital of Soochow University , Suzhou, Jiangsu, China
          Author notes
          CONTACT Liangliang Wang wll234@ 123456163.com
          Article
          PMC6527275 PMC6527275 6527275 1593644
          10.1080/15384101.2019.1593644
          6527275
          30892130
          4d609545-8192-4ff7-bc7c-4c8cb85a91fe
          © 2019 Informa UK Limited, trading as Taylor & Francis Group
          History
          : 18 September 2018
          : 11 February 2019
          : 15 February 2019
          Page count
          Figures: 6, References: 24, Pages: 9
          Funding
          This paper was not funded.
          Categories
          Research Paper

          ubiquitination,long non-coding RNA GAS5,COX-2,chronic non-bacterial prostatitis,Cell proliferation

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