Brendan J Loftus 1 , Eula Fung , Paola Roncaglia , Don Rowley , Paolo Amedeo , Dan Bruno , Jessica Vamathevan , Molly Miranda , Iain J Anderson , James A Fraser , Jonathan E Allen , Ian E Bosdet , Michael R Brent , Readman Chiu , Tamara L Doering , Maureen J Donlin , Cletus A D'Souza , Deborah S Fox , Viktoriya Grinberg , Jianmin Fu , Marilyn Fukushima , Brian J Haas , James C Huang , Guilhem Janbon , Steven J M Jones , Hean L Koo , Martin I Krzywinski , June K Kwon-Chung , Klaus B Lengeler , Rama Maiti , Marco A Marra , Robert E Marra , Carrie A Mathewson , Thomas G Mitchell , Mihaela Pertea , Florenta R Riggs , Steven L Salzberg , Jacqueline E Schein , Alla Shvartsbeyn , Heesun Shin , Martin Shumway , Charles A Specht , Bernard B Suh , Aaron Tenney , Terry R Utterback , Brian L Wickes , Jennifer R Wortman , Natasja H Wye , James W Kronstad , Jennifer K Lodge , Joseph Heitman , Ronald W Davis , Claire M Fraser , Richard W Hyman
Feb 25 2005
Cryptococcus neoformans is a basidiomycetous yeast ubiquitous in the environment, a model for fungal pathogenesis, and an opportunistic human pathogen of global importance. We have sequenced its approximately 20-megabase genome, which contains approximately 6500 intron-rich gene structures and encodes a transcriptome abundant in alternatively spliced and antisense messages. The genome is rich in transposons, many of which cluster at candidate centromeric regions. The presence of these transposons may drive karyotype instability and phenotypic variation. C. neoformans encodes unique genes that may contribute to its unusual virulence properties, and comparison of two phenotypically distinct strains reveals variation in gene content in addition to sequence polymorphisms between the genomes.