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      The effect of hyaluronan-based delivery of stromal cell-derived factor-1 on the recruitment of MSCs in degenerating intervertebral discs.

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          Abstract

          Intervertebral disc (IVD) degeneration is the leading cause of low back pain and disability in the active population. Transplantation of mesenchymal stem cells (MSCs) in a hydrogel carrier can induce regenerative effects in degenerated IVDs. Moreover, it was found that degenerative discs release chemoattractants effective in MSC recruitment. Based on these findings, we hypothesized that an injectable hydrogel that can enhance the number of migrated MSCs in the IVD and provide a suitable matrix for their survival and differentiation would be ideal. The purpose of this study was to evaluate the potential of a thermoreversible hyaluronan-poly(N-isopropylacrylamide) (HAP) hydrogel as chemoattractant delivery system to recruit human MSCs in degenerative IVDs. The results demonstrate that HAP hydrogels containing stromal cell derived factor-1 (SDF-1) significantly increased the number of MSCs migrating into nucleotomized discs compared with discs treated with only HAP or SDF-1 in solution. HAP hydrogels releasing SDF-1 enhanced both the number of recruited cells and their migration distance in the IVD tissue. Furthermore, this phenomenon was dependent on MSC donor age. In conclusion, HAP SDF-1 is effective for the recruitment of stem cells in the IVD, thus opening new possibilities for the development of regenerative therapies based on endogenous cell migration.

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          Author and article information

          Journal
          Biomaterials
          Biomaterials
          1878-5905
          0142-9612
          Sep 2014
          : 35
          : 28
          Affiliations
          [1 ] INEB - Instituto de Engenharia Biomédica, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; ICBAS - Instituto de Ciências Biomédicas Abel Salazar da Universidade do Porto, Rua Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.
          [2 ] INEB - Instituto de Engenharia Biomédica, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal.
          [3 ] AO Research Institute Davos, Clavadelerstrasse 8, 7270 Davos, Switzerland.
          [4 ] AO Research Institute Davos, Clavadelerstrasse 8, 7270 Davos, Switzerland. Electronic address: sibylle.grad@aofoundation.org.
          Article
          S0142-9612(14)00693-0
          10.1016/j.biomaterials.2014.06.017
          24969636
          4e4e9d77-d992-49d6-ad15-ab995c56081c
          Copyright © 2014 Elsevier Ltd. All rights reserved.
          History

          Intervertebral disc,Mesenchymal stem cells,Recruitment,Stromal cell derived factor-1,Thermoreversible hyaluronan hydrogel

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