N-Glycosylated Variants of Growth Hormone in Human Pituitary Extracts

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Abstract

This study was designed to investigate the existence, in human pituitary extracts, of growth hormone (GH) variants not encoded by the hGH-N gene. Using anion exchange-fast protein liquid chromatography followed by SDS-PAGE, we isolated several basic forms of pituitary GH. Incubation of these basic forms with endoglycosidase F/N-glycosidase F revealed that two of them (about 34 and 12 kD) were N-glycosylated. In contrast, no changes were found when samples were incubated with the O-linked glycosylation-specific O-glycosidase. Since the GH-N molecule lacks consensus sequences for N-linked glycosylation, our findings suggest that GH genes other than hGH-N are expressed in the human pituitary gland.

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Journal ID (publisher-id): HRE

Journal ID (nlm-ta): Horm Res Paediatr

Journal ID (doi): 10.1159/issn.1663-2818

Title: Hormone Research in Paediatrics

Publisher: S. Karger AG

ISSN (Print): 1663-2818

ISSN (Electronic): 1663-2826

Publication date Subscription-year: 2000

Publication date (Print): 2000

Publication date (Electronic): 11 August 2000

Volume: 53

Issue: 1

Pages: 40-45

Affiliations

Departamento de Fisiología, Facultad de Medicina, Santiago de Compostela, España

Article

Publisher ID: 23512 Other ID: Horm Res 2000;53:40–45

DOI: 10.1159/000023512

PubMed ID: 10965220

SO-VID: 4e6cf932-4fd2-4486-8905-93a9389dbde4

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N-Glycosylated Variants of Growth Hormone in Human Pituitary Extracts

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Karger: Endocrinology

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