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      N-Glycosylated Variants of Growth Hormone in Human Pituitary Extracts

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          This study was designed to investigate the existence, in human pituitary extracts, of growth hormone (GH) variants not encoded by the hGH-N gene. Using anion exchange-fast protein liquid chromatography followed by SDS-PAGE, we isolated several basic forms of pituitary GH. Incubation of these basic forms with endoglycosidase F/N-glycosidase F revealed that two of them (about 34 and 12 kD) were N-glycosylated. In contrast, no changes were found when samples were incubated with the O-linked glycosylation-specific O-glycosidase. Since the GH-N molecule lacks consensus sequences for N-linked glycosylation, our findings suggest that GH genes other than hGH-N are expressed in the human pituitary gland.

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          Author and article information

          Horm Res Paediatr
          Hormone Research in Paediatrics
          S. Karger AG
          11 August 2000
          : 53
          : 1
          : 40-45
          Departamento de Fisiología, Facultad de Medicina, Santiago de Compostela, España
          23512 Horm Res 2000;53:40–45
          © 2000 S. Karger AG, Basel

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          Figures: 4, References: 19, Pages: 6
          Original Paper


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