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      Effects of carcass weight increase on meat quality and sensory properties of pork loin

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          Abstract

          The objective of this study was to investigate the influence of increasing carcass weight (CW) on meat quality traits including meat color, water-holding capacity (WHC), tenderness, backfat thickness and intramuscular fat (IMF) content of pork loin. A total of 96 pork carcasses (48 LYD [Landrace × Yorkshire × Duroc] barrows and gilts) were selected at a commercial slaughterhouse. Each gender had commercial CW (≤ 90 kg), heavy CW (91–100 kg) and very heavy CW (> 100 kg) (16 carcasses from each CW group). Loin cuts ( Longissimus lumborum) were excised to investigate meat color (CIE L*a*b*), drip loss, cooking loss, released water, Warner-Bratzler shear force (WBSF), and IMF content. Backfat thickness and IMF content of pork loin samples were significantly ( p < 0.05) increased with increasing CW, although there was no significant difference in ultimate pH (pHu). CIE a* increased significantly ( p < 0.05) with increasing CW, while there were no significant differences in CIE L* or CIE b* among CW groups. Although all WHC measures showed no significant differences among CW groups, WBSF increased significantly ( p < 0.01) with increasing CW. Sensory flavor score was significantly increased while panel score for tenderness was decreased significantly ( p < 0.001) with increasing CW. Consequently, CW had a positive correlation with flavor but negative correlation with tenderness. These results indicate that the increased IMF content improves flavor, juiciness and palatability, although tenderness deteriorates with increasing CW.

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          A simple method for the isolation and purification of total lipides from animal tissues.

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            The relationship of sarcoplasmic and myofibrillar protein solubility to colour and water-holding capacity in porcine longissimus muscle.

            In order to investigate the relationship of sarcoplasmic and myofibrillar protein solubility to colour and water-holding capacity (WHC) in pork, 60 loins were selected to represent the quality classes: PSE (pale, soft, exudative), RSE (reddish-pink, soft, exudative), RFN (reddish-pink, firm, non-exudative) and DFD (dark, firm, dry). PSE samples exhibited lower (p<0.05) protein solubility (sarcoplasmic, myofibrillar and total) compared to the other quality classes. RSE samples exhibited lower (p<0.05) sarcoplasmic protein solubility compared to DFD samples. RSE, RFN and DFD samples had similar myofibrillar and total protein solubilities. Sarcoplasmic protein solubility explained 71% of the variation in lightness with a linear decrease in L* value. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) gels of the sarcoplasmic and myofibrillar samples distinctly showed the association of some sarcoplasmic proteins with the myofibrillar protein fractions in PSE and RSE samples. The sarcoplasmic proteins which precipitated were phosphorylase, creatine kinase, triose phosphate isomerase and myokinase for PSE and phosphorylase for RSE samples. Pork colour is highly correlated with precipitation of sarcoplasmic proteins while WHC is affected by denaturation of myofibrillar proteins (PSE samples) and lower ultimate pH (PSE and RSE samples).
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              Correlations among selected pork quality traits.

              Establishing relationships among specific quality traits is important if significant progress toward developing improved pork quality is to be realized. As part of a study to examine the individual effects of genes on meat quality traits in pigs, a three-generation resource family was developed. Two Berkshire sires and nine Yorkshire dams were used to produce nine F1 litters. Sixty-five matings were made from the F1 litters to produce four sets of F2 offspring, for a total of 525 F2 animals used in the study. These F2 animals were slaughtered at a commercial facility upon reaching approximately 110 kg. Carcass composition traits, pH measurements, and subjective quality scores were made at 24 h postmortem. Loin samples (n = 525) were collected at 48 h postmortem, and meat quality traits were evaluated. These traits included pH (48 h), Hunter L-values, drip loss, glycolytic potential, ratio of type IIa/IIb myosin heavy chains (IIa/IIb), total lipid, instrumental measures of tenderness using the Star Probe attachment of the Instron, cook loss measurements, and sensory evaluations. Significant phenotypic correlations were found between many carcass, instrumental, and biochemical measurements, and sensory quality traits. Star Probe measurements were significantly correlated with drip loss (0.29), glycolytic potential (0.30), pH (-0.29), total lipid (-0.14), and Hunter L-values (0.28). Drip loss was significantly correlated with glycolytic potential (0.36), pH (-0.28), IIa/IIb (-0.10), and Hunter L-values (0.33). Hunter L-values were also significantly correlated with total lipid (0.33) and IIa/ IIb (-0.11). Sensory tenderness, flavor, and off-flavor scores were significantly correlated with drip loss, pH, and glycolytic potential measurements. Marbling score, total lipid, and drip loss were not significantly correlated with sensory juiciness scores, but cooking loss was. Marbling and total lipid were significantly correlated with firmness scores (0.37 and 0.31, respectively). Taken together, the data in this study suggest that changes in some meat quality traits can affect many other meat quality attributes. The correlations yield information that could aid in directing future studies aimed at understanding the underlying biological mechanisms behind the development of many quality traits.
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                Author and article information

                Journal
                J Anim Sci Technol
                J Anim Sci Technol
                J Anim Sci Technol
                jast
                Journal of Animal Science and Technology
                Korean Society of Animal Sciences and Technology
                2672-0191
                2055-0391
                September 2020
                30 September 2020
                : 62
                : 5
                : 753-760
                Affiliations
                [1 ]Institute of Agriculture & Life Science, Gyeongsang National University, Jinju 52852, Korea
                [2 ]Division of Applied Life Science (BK21+), Gyeongsang National University, Jinju 52852, Korea
                Author notes
                [* ]Corresponding author: Seon-Tea Joo, Division of Applied Life Science (BK21 +), Gyeongsang National University, Jinju 52828, Korea. Tel: +82-55-772-1943 E-mail: stjoo@ 123456gnu.ac.kr

                #These authors contributed equally to this work.

                Author information
                https://orcid.org/0000-0003-3687-3535
                https://orcid.org/0000-0001-6030-0601
                https://orcid.org/0000-0002-3467-7349
                https://orcid.org/0000-0002-5483-2828
                Article
                jast-62-5-753
                10.5187/jast.2020.62.5.753
                7553834
                33089239
                4eda6ba2-29c2-4e95-be35-83f3e688a5ae
                © Copyright 2020 Korean Society of Animal Science and Technology

                This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

                History
                : 13 August 2020
                : 10 September 2020
                : 14 September 2020
                Funding
                Funded by: CrossRef http://dx.doi.org/10.13039/501100003725, National Research Foundation of Korea;
                Award ID: 2019R1I1A1A01061707
                Categories
                Research Article
                Custom metadata
                2020-10-31

                pork carcass,carcass weight,heavy slaughter weight,pork quality

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