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      Intrinsic Pulsatility of Luteinizing Hormone Release from the Human Pituitary in vitro

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          Abstract

          An in vitro perifusion system was used to investigate the spontaneous luteinizing hormone (LH) release from 10 human fetal (21–23 weeks of gestation) and 1 adult female pituitaries. The pattern of LH release from fetal pituitaries (n = 6) exhibited a remarkable pulsatile character with a mean (±SE) pulse interval of 12.7 + 1.7 min. The mean pulse amplitude was 5.2 ± 0.9 mlU with a nadir to peak increment of 69.5 ± 6.4%. The mean LH release rate was 12.3 ± 3.3 mIU/2 min. Blockade of calcium activity with 0.1 mM verapamil and 4 mM EGTA suppressed the frequency (from 1 pulse/12–20 min to 1 pulse/50–100 min) and amplitude (from 5.4–5.7 mlU to 1.4–2.1 mlU) of this spontaneous pulsatile LH release (n = 2). Administration of 8 nMgonadotropin-releasing hormone induced 255 and 954% increases in LH secretion (n = 2). Each quarter of an adult human pituitary also secreted LH in a pulsatile fashion, with a pulse interval of 15.2 ± 5.6 min, a pulse amplitude of 5.4 ± 0.6 mlU, a nadir to peak increment of 67.5 ± 5.2%, and an overall release rate of 14.8 ± 0.9 mIU/2 min. These studies demonstrate that LH release from the isolated human pituitary in vitro is characterized by high-frequency/low-amplitude pulses, independent of hypothalamic stimulation. Accordingly, this spontaneous calcium-mediated pulsatile LH release apparently reflects the activity of an intrinsic intrapituitary pulse-generating mechanism.

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          Author and article information

          Journal
          NEN
          Neuroendocrinology
          10.1159/issn.0028-3835
          Neuroendocrinology
          S. Karger AG
          0028-3835
          1423-0194
          1987
          1987
          02 April 2008
          : 45
          : 5
          : 402-406
          Affiliations
          Department of Reproductive Medicine, School of Medicine, University of California, San Diego, La Jolla, Calif., USA
          Article
          124765 Neuroendocrinology 1987;45:402–406
          10.1159/000124765
          3587522
          © 1987 S. Karger AG, Basel

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          Page count
          Pages: 5
          Categories
          Original Paper

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