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A large genome center's improvements to the Illumina sequencing system
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Abstract
The Wellcome Trust Sanger Institute is one of the world's largest genome centers,
and a substantial amount of our sequencing is performed with 'next-generation' massively
parallel sequencing technologies: in June 2008 the quantity of purity-filtered sequence
data generated by our Genome Analyzer (Illumina) platforms reached 1 terabase, and
our average weekly Illumina production output is currently 64 gigabases. Here we describe
a set of improvements we have made to the standard Illumina protocols to make the
library preparation more reliable in a high-throughput environment, to reduce bias,
tighten insert size distribution and reliably obtain high yields of data.