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      Soluble cytokine receptors are present in normal human urine

      research-article
      The Journal of Experimental Medicine
      The Rockefeller University Press

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          Abstract

          Affinity chromatography of crude human urinary proteins on either human rIL-6, human rIFN-gamma, or anti-IFN-gamma-R mAb yielded the two respective soluble receptors in significant quantities. A single sequence of 30 amino acid residues was obtained by NH2-terminal microsequencing of the protein peak purified in tandem by affinity chromatography on an IL-6 column and reversed-phase HPLC. This sequence was identical to the predicted NH2-terminal sequence of IL-6-R as previously reported. Analysis of the eluted proteins from both IFN- gamma and anti-IFN-gamma-R columns by inhibition of solid phase RIA, ELISA, SDS-PAGE, and Western blotting proved the existence of soluble IFN-gamma-R in normal urine. Our finding, together with the already known presence of urinary TNF binding proteins and a soluble IL-2-R both in plasma and in urine, indicates that release of soluble cytokine receptors into body fluids is a general phenomenon that occurs under normal physiological conditions.

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          Author and article information

          Journal
          J Exp Med
          The Journal of Experimental Medicine
          The Rockefeller University Press
          0022-1007
          1540-9538
          1 October 1989
          : 170
          : 4
          : 1409-1414
          Article
          90010793
          10.1084/jem.170.4.1409
          2189483
          2529343
          4f8322d7-5c37-49a2-b431-1c55e7eb34af
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          Medicine
          Medicine

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