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      Growth of Cultured Corneal Endothelial Cells onto a Vitreous Carbon Matrix

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          Fourth passage cells of a rabbit corneal endothelial line were grown for 1 week in flasks containing pieces of a reticulated vitreous carbon matrix. The rate of cell growth in flasks containing the matrix was consistent with that in control flasks. Small fragments of the vitreous carbon material lying on the flask floor were covered by the monolayers as the cells grew to confluency. Vertical growth of cells onto larger pieces of the matrix proceeded in a staged fashion with maximum cell density on pieces of the matrix closest to the floor of the flask. As defined by scanning electron microscopy, cell growth occurred to a level at least 600 µm above the floor of the flask and the confluent monolayer. This novel culture procedure should be a model situation for study of many different aspects of the in vitro capabilities of corneal endothelial cells.

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          Author and article information

          Ophthalmic Res
          Ophthalmic Research
          S. Karger AG
          04 December 2009
          : 15
          : 2
          : 116-120
          Division of Ophthalmology, School of Medicine, University of California at San Diego and the VA Medical Center, San Diego, Calif. USA
          265244 Ophthalmic Res 1983;15:116–120
          © 1983 S. Karger AG, Basel

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          Pages: 5
          Original Paper


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