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      The effect of inoculum age and solid versus liquid propagation on inoculum quality of an industrial Bacillus licheniformis strain.

      1 , , ,
      FEMS microbiology letters
      Elsevier BV

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          Abstract

          Shorter lag phases were obtained in cultivations of Bacillus licheniformis using early-compared to late-stationary growth phase inocula and using liquid versus solid propagation medium. Flow cytometry and fluorescence ratio imaging microscopy (FRIM) after staining with 5(6)-carboxyfluorescein diacetate succinimidyl ester (CFDA-SE), confirmed that liquid early-stationary growth phase inoculum had a higher vitality and was more homogeneous than solid late-stationary growth phase inoculum. DNA-microarray analyses indicated that liquid early-stationary growth phase inoculum was in a more active state in terms of cell multiplication whereas solid late-stationary growth phase inoculum was induced to some spore formation potentially causing delayed growth initiation.

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          Author and article information

          Journal
          FEMS Microbiol Lett
          FEMS microbiology letters
          Elsevier BV
          0378-1097
          0378-1097
          Jul 01 2004
          : 236
          : 1
          Affiliations
          [1 ] Novozymes A/S, Bagsvaerd, Denmark. tho@kvl.dk
          Article
          S0378109704003878
          10.1016/j.femsle.2004.05.035
          15212804
          4fea840c-0037-4bf4-ae4d-716cc29bb913
          History

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