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      Uso de PEG4000 para evaluar la influencia de los polifenoles en la producción de gas in vitro con heces vacunas como inóculo Translated title: Use of PEG400 to assess the influence of polyphenols on in vitro gas production with bovine feces as inoculum

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          Abstract

          En la búsqueda de técnicas analíticas que permitan obtener resultados confiables para la evaluación de materias primas y/o forrajes a ser usados en la alimentación de rumiantes, la medición de la producción de gas in vitro con el uso de heces vacunas puede ser un procedimiento de elección para predecir la digestibilidad, sin agresión alguna a dichos animales. Aplicando esta técnica, se estudiaron muestras de Chamaecrista lineada, Desmodium barbatum, Desmodium incanum, Dichrostachys cinerea, Leucaena leucocephala y Stylosanthes viscosa. Se evaluó la influencia de la adición de PEG4000 en la producción de gas y contrarrestó su efecto con la cantidad de polifenoles extractables presentes en las muestras. La adición del polímero mejoró (P<0,01) la producción de gas (mL/200 mg MS) para Ch. lineada (8,5 a 12,3), D. cirenea (17,3 a 27,1) y L. leucocephala (16,8 a 20,0). En las leguminosas estudiadas, existió correlación positiva (r² = 0,94) entre la mejora producida por el PEG en la producción de gas y la concentración de polifenoles totales.

          Translated abstract

          In the search of analytical techniques to achieve reliable results in assessing animal feed raw materials and/or forages, in vitro gas production using bovine feces can be a selected procedure to predict digestibility, without any aggression to the animals. Applying such technique, samples of Chamaecrista lineada, Desmodium barbatum, Desmodium incanum, Dichrostachys cinerea, Leucaena leucocephala, and Stylosanthes viscosa were evaluated. The correlation between the influence of PEG4000 on gas production and the concentration of extractable polyphenols was evaluated. The addition of PEG significantly (P<0.01) improved in vitro gas production (mL/200 mg DM) of Ch. lineada (8.5 to 12.3), D. cirenea (17.3 to 27.1), and L. leucocephala (16.8 to 20.0). In the evaluated legumes, there was a positive correlation (r² = 0.94) between the improvement in gas production because of PEG and the concentration of polyphenols.

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          Formation of complexes between polyvinyl pyrrolidones or polyethylene glycols and tannins, and their implication in gas production and true digestibility in in vitro techniques.

          Various tannin-complexing agents have been used to study the potential adverse effects of tannins on rumen metabolism. Using a method based on turbidity formation, the binding of various tannin-complexing agents (polyvinyl polypyrrolidone (PVPP), polyethylene glycol (PEG) of molecular weights 2000 to 35,000, and polyvinyl pyrrolidone (PVP) of molecular weight 10,000, 40,000 and 360,000) to tannins (tannic acid, purified tannins from quebracho (Aspidosperma quebracho) and leaves of trees and shrubs (Acioa barteri, Dichostachys cinerea, Guiera senegalensis, Piliostigma reticulatum)) was investigated at different pH values. The binding of all the tannins with PVPP was highest at pH 3-4 and lowest at pH 7. For all the pH range (3-7) studied, the binding of PEG was higher than that of PVP. For all the tannins except tannic acid, the binding to PVP was the same from pH 4.7 to 7. Similar results were observed for the PEG of molecular weight 6000 or higher for all the tannins except quebracho tannins for which the binding increased as the pH increased from 3 to 7. The binding with PEG 2000 decreased to a greater extent as the pH reached near neutral and for PEG 4000 this decrease was slightly lower. Addition of these tannin-complexing agents to the in vitro gas system resulted in higher gas production from tannin-rich feeds (increase varied from 0 to 135%). The PEG were the most effective followed by PVP and PVPP. The PEG 35,000 was least effective. The efficiency of other PEG was similar. The PEG 6000 was preferred to PEG 2000 or 4000 as its binding to tannins was higher at near neutral pH values. The gas production increased with an increase in the amount of PEG 6000 up to 0.6 g/40 ml rumen-fluid-containing medium containing 0.5 g tannin-rich feed, beyond which no increase was observed. The percentage increase in gas value at 24 h fermentation correlated significantly with tannin values, the highest correlation (r 0.95) being with protein precipitation capacity of tannins. The increase in gas production was associated with higher production of short-chain fatty acids with little change in their molar proportions, suggesting an increase in organic matter digestibility by inclusion of the PEG in tannin-rich feeds. However, apparent and true digestibilities were lower on addition of the PEG, due to the presence of PEG-tannin complexes in the residues. The use of this bioassay (percentage increase in gas production in the presence of PEG 6000) along with other tannin assays would provide a better insight into the nutritional significance of tannins.
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            In vitro fermentation and microbial protein synthesis of some browse tree leaves with or without addition of polyethylene glycol

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              The effectiveness of adapted rumen fluid versus PEG to ferment tannin-containing substrates in vitro

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                Author and article information

                Journal
                zt
                Zootecnia Tropical
                Zootecnia Trop.
                Instituto Nacional de Investigaciones Agricolas INIA, Maracay, Venezuela. (Maracay, Aragua, Venezuela )
                0798-7269
                September 2008
                : 26
                : 3
                : 261-264
                Affiliations
                [01] orgnameUniversidad de Camagüey orgdiv1Facultad de Ciencias Agropecuarias Cuba
                Article
                S0798-72692008000300022 S0798-7269(08)02600322
                4ff23cba-3d59-4e28-a4da-ed2756ca3e28

                http://creativecommons.org/licenses/by/4.0/

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                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 6, Pages: 4
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                SciELO Venezuela

                Self URI: Texto completo solamente en formato PDF (ES)
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                Artículos Científicos

                legumes,polyphenols,PEG,feces,in vitro gas,leguminosas,polifenoles,heces,gas in vitro

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