Serotonin is required for pharyngeal arch morphogenesis in zebrafish

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Abstract

Serotonin (5-HT) is not only a neurotransmitter but also a mediator of developmental processes in vertebrates. In this study, we analyzed the importance of 5-HT during zebrafish development. The expression patterns of three zebrafish tryptophan hydroxylase isoforms (Tph1A, Tph1B, Tph2), the rate-limiting enzymes in 5-HT synthesis, were analyzed and compared to the appearance and distribution of 5-HT. 5-HT was found in the raphe nuclei correlating with tph2 expression and in the pineal gland correlating with tph1a and tph2 expressions. Tph2-deficient fish generated with antisense morpholino oligonucleotides exhibited morphogenesis defects during pharyngeal arch development. The correct specification of neural crest (NC) cells was not affected in tph2 morphants as shown by the expression of early markers, but the survival and differentiation of pharyngeal arch progenitor cells were impaired. An organizing role of 5-HT in pharyngeal arch morphogenesis was suggested by a highly regular pattern of 5-HT positive cells in this tissue. Moreover, the 5-HT2B receptor was expressed in the pharyngeal arches and its pharmacological inhibition also induced defects in pharyngeal arch morphogenesis. These results support an important role of Tph2-derived serotonin as a morphogenetic factor in the development of NC-derived tissues.

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Author and article information

Contributors

Saleh Bashammakh: (View ORCID Profile)

Journal

Journal ID (publisher-id): SOR-LIFE

Title: ScienceOpen Research

Publisher: ScienceOpen

ISSN (Electronic): 2199-1006

Publication date: 22 December 2014

Volume: 0 (ID: 501d70cc-221a-444f-a69e-b74f39809599 )

Issue: 0

Pages: 1-9

Affiliations

[1 ]Max-Delbrück-Center for Molecular Medicine (MDC), Berlin, Germany

Author notes

[* ]Corresponding author's e-mail address: sbashammakh@ 123456hotmail.de

Article

Publisher ID: 2326:XE

DOI: 10.14293/S2199-1006.1.SOR-LIFE.AWPDLZ.v1

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This work has been published open access under Creative Commons Attribution License CC BY 4.0 , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Conditions, terms of use and publishing policy can be found at www.scienceopen.com .