Development of a smartphone-based rapid dual fluorescent diagnostic system for the simultaneous detection of influenza A and H5 subtype in avian influenza A-infected patients

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Abstract

Accurate and rapid diagnosis of highly pathogenic avian influenza A H5N1 is of critical importance for the effective clinical management of patients. Here, we developed a rapid and simultaneous detection toolkit for influenza A H5 subtype viruses in human samples based on a bioconjugate of quantum dots (QDs) assembly and a smartphone-based rapid dual fluorescent diagnostic system (SRDFDS).

Methods: Two types of QDs were assembled on a latex bead to enhance the detection sensitivity and specificity of influenza A infection (QD580) and H5 subtype (QD650). The dual signals of influenza A and H5 subtype of H5N1-infected patients were detected simultaneously and quantified separately by SRDFDS equipped with two emission filters.

Results: Our results showed a high sensitivity of 92.86% (13/14) and 78.57% (11/14), and a specificity of 100% (38/38, P < 0.0001) and 97.37% (37/38) for influenza A and H5 subtype detection, respectively.

Conclusion: Therefore, our multiplex QD bioconjugates and SRDFDS-based influenza virus detection toolkit potentially provide accurate and meaningful diagnosis information with improved detection accuracies and sensitivities for H5N1 patients.

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Large-scale synthesis of nearly monodisperse CdSe/CdS core/shell nanocrystals using air-stable reagents via successive ion layer adsorption and reaction.

J. Jack Li, Y Andrew Wang, Wenzhuo Guo … (2003)

Successive ion layer adsorption and reaction (SILAR) originally developed for the deposition of thin films on solid substrates from solution baths is introduced as a technique for the growth of high-quality core/shell nanocrystals of compound semiconductors. The growth of the shell was designed to grow one monolayer at a time by alternating injections of air-stable and inexpensive cationic and anionic precursors into the reaction mixture with core nanocrystals. The principles of SILAR were demonstrated by the CdSe/CdS core/shell model system using its shell-thickness-dependent optical spectra as the probes with CdO and elemental S as the precursors. For this reaction system, a relatively high temperature, about 220-240 degrees C, was found to be essential for SILAR to fully occur. The synthesis can be readily performed on a multigram scale. The size distribution of the core/shell nanocrystals was maintained even after five monolayers of CdS shell (equivalent to about 10 times volume increase for a 3.5 nm CdSe nanocrystal) were grown onto the core nanocrystals. The epitaxial growth of the core/shell structures was verified by optical spectroscopy, TEM, XRD, and XPS. The photoluminescence quantum yield (PL QY) of the as-prepared CdSe/CdS core/shell nanocrystals ranged from 20% to 40%, and the PL full-width at half-maximum (fwhm) was maintained between 23 and 26 nm, even for those nanocrystals for which the UV-vis and PL peaks red-shifted by about 50 nm from that of the core nanocrystals. Several types of brightening phenomena were observed, some of which can further boost the PL QY of the core/shell nanocrystals. The CdSe/CdS core/shell nanocrystals were found to be superior in comparison to the highly luminescent CdSe plain core nanocrystals. The SILAR technique reported here can also be used for the growth of complex colloidal semiconductor nanostructures, such as quantum shells and colloidal quantum wells.

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Multiplexed Point-of-Care Testing – xPOCT

Can Dincer, Richard Bruch, André Kling … (2017)

Multiplexed point-of-care testing (xPOCT), which is simultaneous on-site detection of different analytes from a single specimen, has recently gained increasing importance for clinical diagnostics, with emerging applications in resource-limited settings (such as in the developing world, in doctors’ offices, or directly at home). Nevertheless, only single-analyte approaches are typically considered as the major paradigm in many reviews of point-of-care testing. Here, we comprehensively review the present diagnostic systems and techniques for xPOCT applications. Different multiplexing technologies (e.g., bead- or array-based systems) are considered along with their detection methods (e.g., electrochemical or optical). We also address the unmet needs and challenges of xPOCT. Finally, we critically summarize the in-field applicability and the future perspectives of the presented approaches.

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Rapid and sensitive detection of protein biomarker using a portable fluorescence biosensor based on quantum dots and a lateral flow test strip.

Zhiwen Tang, Sheng-Jun Wang, Joel G Pounds … (2010)

A portable fluorescence biosensor with rapid and ultrasensitive response for protein biomarker has been built up with quantum dots and a lateral flow test strip. The superior signal brightness and high photostability of quantum dots are combined with the promising advantages of a lateral flow test strip and result in high sensitivity and selectivity and speed for protein detection. Nitrated ceruloplasmin, a significant biomarker for cardiovascular disease, lung cancer, and stress response to smoking, was used as model protein biomarker to demonstrate the good performances of this proposed quantum dot-based lateral flow test strip. Quantitative detection of nitrated ceruloplasmin was realized by recording the fluorescence intensity of quantum dots captured on the test line. Under optimal conditions, this portable fluorescence biosensor displays rapid responses for nitrated ceruloplasmin with the concentration as low as 1 ng/mL. Furthermore, the biosensor was successfully utilized for spiked human plasma sample detection in a wide dynamic range with a detection limit of 8 ng/mL (S/N = 3). The results demonstrate that the quantum dot-based lateral flow test strip is capable of rapid, sensitive, and quantitative detection of nitrated ceruloplasmin and hold a great promise for point-of-care and in field analysis of other protein biomarkers.

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Author and article information

Journal

Journal ID (nlm-ta): Theranostics

Journal ID (iso-abbrev): Theranostics

Journal ID (publisher-id): thno

Title: Theranostics

Publisher: Ivyspring International Publisher (Sydney )

ISSN (Electronic): 1838-7640

Publication date Collection: 2018

Publication date (Electronic): 29 November 2018

Volume: 8

Issue: 22

Pages: 6132-6148

Affiliations

[1 ]Zoonosis Research Center, Department of Infection Biology, School of Medicine, Wonkwang University, Iksan, 570-749, Republic of Korea

[2 ]Gordon Center for Medical Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA

[3 ]National Institute of Hygiene and Epidemiology, No 1- Yersin street, Hanoi, Vietnam

[4 ]School of Electrical Engineering, Korea Advanced Institute of Science and Technology, Daejeon, 305-338, Republic of Korea

[5 ]Department of Microbiology, Ajou University School of Medicine, and Department of Biomedical Science, Ajou University Graduate School of Medicine, Suwon 16499, Republic of Korea

[6 ]GenBody Inc., 3-18, Eopseong 2-gil, Seobuk-gu, Cheonan, 31077, Republic of Korea

Author notes

✉ Corresponding author: Hyun Park, MD, Ph, D: hyunpk@ 123456wku.ac.kr

^* These authors contributed equally to this work.

Competing Interests: The authors have declared that no competing interest exists.

Article

Publisher ID: thnov08p6132

DOI: 10.7150/thno.28027

PMC ID: 6299699

PubMed ID: 30613288

SO-VID: 5056d694-1c73-4fc2-af94-8943a68e57db

License:

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license ( https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions.

History

Date received : 22 June 2018

Date accepted : 30 October 2018

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Development of a smartphone-based rapid dual fluorescent diagnostic system for the simultaneous detection of influenza A and H5 subtype in avian influenza A-infected patients

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Most cited references 47

Large-scale synthesis of nearly monodisperse CdSe/CdS core/shell nanocrystals using air-stable reagents via successive ion layer adsorption and reaction.

Multiplexed Point-of-Care Testing – xPOCT

Rapid and sensitive detection of protein biomarker using a portable fluorescence biosensor based on quantum dots and a lateral flow test strip.

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Cited by 9

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