Single Cell Raman Spectroscopy Deuterium Isotope Probing for Rapid Antimicrobial Susceptibility Test of <i>Elizabethkingia</i> spp.

There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

Abstract

Nosocomial infection by multi-drug resistance Elizabethkingia spp. is an emerging concern with severe clinical consequences, particularly in immunocompromised individuals and infants. Efficient control of this infection requires quick and reliable methods to determine the appropriate drugs for treatment. In this study, a total of 31 Elizabethkingia spp., including two standard strains (ATCC 13253 and FMS-007) and 29 clinical isolates obtained from hospitals in China were subjected to single cell Raman spectroscopy analysis coupled with deuterium probing (single cell Raman-DIP). The results demonstrated that single cell Raman-DIP could determine antimicrobial susceptibility of Elizabethkingia spp. in 4 h, only one third of the time required by standard broth microdilution method. The method could be integrated into current clinical protocol for sepsis and halve the report time. The study also confirmed that minocycline and levofloxacin are the first-line antimicrobials for Elizabethkingia spp. infection.

Related collections

Most cited references 47

Record: found
Abstract: found
Article: not found

The antibiotic resistance crisis: part 1: causes and threats.

C Lee Ventola (2015)

Decades after the first patients were treated with antibiotics, bacterial infections have again become a threat because of the rapid emergence of resistant bacteria-a crisis attributed to abuse of these medications and a lack of new drug development.

0 comments Cited 833 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: found

Is Open Access

Methods for in vitro evaluating antimicrobial activity: A review ☆

Mounyr Balouiri, Moulay Sadiki, Saad Koraichi Ibnsouda (2015)

In recent years, there has been a growing interest in researching and developing new antimicrobial agents from various sources to combat microbial resistance. Therefore, a greater attention has been paid to antimicrobial activity screening and evaluating methods. Several bioassays such as disk-diffusion, well diffusion and broth or agar dilution are well known and commonly used, but others such as flow cytofluorometric and bioluminescent methods are not widely used because they require specified equipment and further evaluation for reproducibility and standardization, even if they can provide rapid results of the antimicrobial agent's effects and a better understanding of their impact on the viability and cell damage inflicted to the tested microorganism. In this review article, an exhaustive list of in vitro antimicrobial susceptibility testing methods and detailed information on their advantages and limitations are reported.

0 comments Cited 707 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: not found

Distinguishing between resistance, tolerance and persistence to antibiotic treatment.

Asher Brauner, Ofer Fridman, Orit Gefen … (2016)

Antibiotic tolerance is associated with the failure of antibiotic treatment and the relapse of many bacterial infections. However, unlike resistance, which is commonly measured using the minimum inhibitory concentration (MIC) metric, tolerance is poorly characterized, owing to the lack of a similar quantitative indicator. This may lead to the misclassification of tolerant strains as resistant, or vice versa, and result in ineffective treatments. In this Opinion article, we describe recent studies of tolerance, resistance and persistence, outlining how a clear and distinct definition for each phenotype can be developed from these findings. We propose a framework for classifying the drug response of bacterial strains according to these definitions that is based on the measurement of the MIC together with a recently defined quantitative indicator of tolerance, the minimum duration for killing (MDK). Finally, we discuss genes that are associated with increased tolerance - the 'tolerome' - as targets for treating tolerant bacterial strains.

0 comments Cited 328 times – based on 0 reviews      Review now

Bookmark

All references

Author and article information

Contributors

Shuying Yuan: URI : http://loop.frontiersin.org/people/1041796/overview

Zhenju Song: URI : http://loop.frontiersin.org/people/842396/overview

Yizhi Song: URI : http://loop.frontiersin.org/people/1629143/overview

Wenhong Zhang: URI : http://loop.frontiersin.org/people/353955/overview

Li Chen: URI : http://loop.frontiersin.org/people/712732/overview

Guiqin Sun: URI : http://loop.frontiersin.org/people/1678089/overview

Journal

Journal ID (nlm-ta): Front Microbiol

Journal ID (iso-abbrev): Front Microbiol

Journal ID (publisher-id): Front. Microbiol.

Title: Frontiers in Microbiology

Publisher: Frontiers Media S.A.

ISSN (Electronic): 1664-302X

Publication date (Electronic): 03 May 2022

Publication date Collection: 2022

Volume: 13

Electronic Location Identifier: 876925

Affiliations

[1] ¹School of Medical Technology and Information Engineering, Zhejiang Chinese Medical University , Hangzhou, China

[2] ²CAS Key Laboratory of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences , Suzhou, China

[3] ³Department of Medical Microbiology and Parasitology, Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, School of Basic Medical Sciences, Fudan University , Shanghai, China

[4] ⁴Department of Emergency Medicine, Zhongshan Hospital, Fudan University , Shanghai, China

[5] ⁵Department of Infectious Diseases, Huashan Hospital, Shanghai Medical College, Fudan University , Shanghai, China

Author notes

Edited by: Weili Hong, Beihang University, China

Reviewed by: Guojun Zhang, Capital Medical University, China; Pu-Ting Dong, The Forsyth Institute, United States

*Correspondence: Yizhi Song, songyz@ 123456sibet.ac.cn

Wenhong Zhang, zhangwenhong@ 123456fudan.edu.cn

Li Chen, lichen_bk@ 123456fudan.edu.cn

Guiqin Sun, sunguiqin2001@ 123456163.com

^†These authors have contributed equally to this work and share first authorship

This article was submitted to Evolutionary and Genomic Microbiology, a section of the journal Frontiers in Microbiology

Article

DOI: 10.3389/fmicb.2022.876925

PMC ID: 9113537

PubMed ID: 35591987

SO-VID: 5057e788-c266-4927-b344-a1091a776640

License:

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

History

Date received : 01 March 2022

Date accepted : 11 April 2022

Page count

Figures: 5, Tables: 3, Equations: 0, References: 48, Pages: 10, Words: 6320

Funding

Funded by: Natural Science Foundation of Zhejiang Province, doi 10.13039/501100004731;

Funded by: National Natural Science Foundation of China, doi 10.13039/501100001809;

Single Cell Raman Spectroscopy Deuterium Isotope Probing for Rapid Antimicrobial Susceptibility Test of Elizabethkingia spp.

Read this article at

Abstract

Related collections

Decoding Infection and Transmission

Most cited references 47

The antibiotic resistance crisis: part 1: causes and threats.

Methods for in vitro evaluating antimicrobial activity: A review ☆

Distinguishing between resistance, tolerance and persistence to antibiotic treatment.

Author and article information

Contributors

Journal

Affiliations

Author notes

Article

History

Page count

Funding

Categories

Comments

Comment on this article

Similar content 556

Cited by 3

Most referenced authors 693