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      A review of experimental aspects of electrochemical immunosensors

      , ,

      Electrochimica Acta

      Elsevier BV

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          Enzyme immunoassay (EIA)/enzyme-linked immunosorbent assay (ELISA).

           M Lequin (2005)
          This brief note addresses the historical background of the invention of the enzyme immunoassay (EIA) and enzyme-linked immunosorbent assay (ELISA). These assays were developed independently and simultaneously by the research group of Peter Perlmann and Eva Engvall at Stockholm University in Sweden and by the research group of Anton Schuurs and Bauke van Weemen in The Netherlands. Today, fully automated instruments in medical laboratories around the world use the immunoassay principle with an enzyme as the reporter label for routine measurements of innumerable analytes in patient samples. The impact of EIA/ELISA is reflected in the overwhelmingly large number of times it has appeared as a keyword in the literature since the 1970s. Clinicians and their patients, medical laboratories, in vitro diagnostics manufacturers, and worldwide healthcare systems owe much to these four inventors.
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            Enzyme immunoassays with special reference to ELISA techniques.

            In this review outlines are given on various types of enzyme immunoassay. The applications to such enzyme immunoassays, especially ELISA, are dealth with in detail. It is concluded that these techniques have high sensitivity and will be suitable in due course as routine laboratory tests.
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              Development and application of lateral flow test strip technology for detection of infectious agents and chemical contaminants: a review.

              Recent progress in the laboratory has been a result of improvements in rapid analytical techniques. An update of the applications of lateral flow tests (also called immunochromatographic assay or test strip) is presented in this review manuscript. We emphasized the description of this technology in the detection of a variety of biological agents and chemical contaminants (e.g. veterinary drugs, toxins and pesticides). It includes outstanding data, such as sample treatment, sensitivity, specificity, accuracy and reproducibility. Lateral flow tests provide advantages in simplicity and rapidity when compared to the conventional detection methods.
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                Author and article information

                Journal
                Electrochimica Acta
                Electrochimica Acta
                Elsevier BV
                00134686
                December 2012
                December 2012
                : 84
                :
                : 74-83
                Article
                10.1016/j.electacta.2012.06.033
                © 2012

                http://www.elsevier.com/tdm/userlicense/1.0/

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