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      Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets

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          Abstract

          Cells, the basic units of biological structure and function, vary broadly in type and state. Single-cell genomics can characterize cell identity and function, but limitations of ease and scale have prevented its broad application. Here we describe Drop-seq, a strategy for quickly profiling thousands of individual cells by separating them into nanoliter-sized aqueous droplets, associating a different barcode with each cell's RNAs, and sequencing them all together. Drop-seq analyzes mRNA transcripts from thousands of individual cells simultaneously while remembering transcripts' cell of origin. We analyzed transcriptomes from 44,808 mouse retinal cells and identified 39 transcriptionally distinct cell populations, creating a molecular atlas of gene expression for known retinal cell classes and novel candidate cell subtypes. Drop-seq will accelerate biological discovery by enabling routine transcriptional profiling at single-cell resolution. VIDEO ABSTRACT.

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          Author and article information

          Journal
          Cell
          Cell
          Elsevier BV
          00928674
          May 2015
          May 2015
          : 161
          : 5
          : 1202-1214
          Article
          10.1016/j.cell.2015.05.002
          26000488
          50e6640f-d67b-4428-8ecd-9891845ea385
          © 2015

          https://www.elsevier.com/tdm/userlicense/1.0/

          https://www.elsevier.com/open-access/userlicense/1.0/

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