31
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Study of telomere length reveals rapid aging of human marrow stromal cells following in vitro expansion.

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Human marrow stromal cells (MSCs) can be isolated from bone marrow and differentiate into multiple tissues in vitro and in vivo. These properties make them promising tools in cell and gene therapy. The lack of a specific MSC marker and the low frequency of MSCs in bone marrow necessitate their isolation by in vitro expansion prior to clinical use. This may severely reduce MSC proliferative capacity to the point that the residual proliferative potential is insufficient to maintain long-term tissue regeneration upon reinfusion. In this study we determined the effect of in vitro expansion on the replicative capacity of MSCs by correlating their rate of telomere loss during in vitro expansion with their behavior in vivo. We report that even protocols that involve minimal expansion induce a rapid aging of MSCs, with losses equivalent to about half their total replicative lifespan.

          Related collections

          Author and article information

          Journal
          Stem Cells
          Stem cells (Dayton, Ohio)
          Wiley
          1066-5099
          1066-5099
          2004
          : 22
          : 5
          Affiliations
          [1 ] Stem Cell Research Group, Giving for Living Postgraduate Centre, Royal Manchester Children's Hospital, M27 4HA, UK.
          Article
          22/5/675
          10.1634/stemcells.22-5-675
          15342932
          50f323da-30c9-4853-8ef3-8994eb96132d
          History

          Comments

          Comment on this article