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Morphological and molecular characterization of three species of Anastrepha Schiner and of Ceratitis capitata (Wiedemann) (Diptera: Tephritidae) Translated title: Caracterização morfológica e molecular de três espécies do gênero Anastrepha Schiner e de Ceratitis capitata (Wiedemann) (Diptera: Tephritidae)

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      Four species of Tephritoidea, three from genus Anastrepha: A. obliqua (Macquart), A. sororcula Zucchi and A. serpentina (Wiedemann), and one from genus Ceratitis, C. capitata (Wiedemann) were compared based on puparium morphology and application of the Heteroduplex Mobility Assay (HMA). Puparia were characterized for the first time using the spiracular posterior plate morphology. Application of HMA allowed the detection of variability in the D2 domain from 28S rRNA gene in all four species (confirmed by sequencing). This is a fast, simple, sensitive and inexpensive assay that was used for the first time in the analysis of two Tephritoidea genera. The detected variability suggests that the tecnique has great potential for rapid determination of infestations with two or more species in the same host fruit.

      Translated abstract

      Foram comparadas com base na morfologia do pupário e na técnica de HMA (Heteroduplex Mobility Assay)) quatro espécies de Tephritoidea, três delas pertencentes ao gênero Anastrepha, A. obliqua (Macquart), A. sororcula Zucchi e A. serpentina (Wiedemann) e uma do gênero Ceratitis, C. capitata (Wiedemann). Os pupários foram caracterizados pela primeira vez com base na morfologia da placa espiracular posterior. A aplicação da técnica de HMA possibilitou a detecção de variabilidade no segmento D2 do gene 28S rRNA nas quatro espécies (confirmada por seqüenciamento). Esta é uma técnica rápida, simples, sensível e barata que é aplicada pela primeira vez na análise de dois gêneros de Tephritoidea. A variabilidade observada sugere grande potencial da técnica no caso da determinação da infestação por duas ou mais espécies num mesmo fruto hospedeiro.

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            Author and article information

            [1 ] Universidade Federal do Rio de Janeiro Brazil
            [2 ] Universidade Federal do Rio de Janeiro Brazil
            Role: ND
            Role: ND
            Role: ND
            Neotropical Entomology
            Neotrop. entomol.
            Sociedade Entomológica do Brasil (Londrina )
            December 2005
            : 34
            : 6
            : 917-925
            S1519-566X2005000600007 10.1590/S1519-566X2005000600007


            Product Information: SciELO Brazil


            HMA, pupário, Tephritoidea, Mosca-das-frutas, puparium, Fruit fly


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