Purification and properties of a novel enzyme fromBacillusspp. T-3040, which catalyses the conversion of dextran to cyclic isomaltooligosaccharides

Three kinds of novel cyclic isomaltooligosaccharides were isolated from the culture broth of a strain of Bacillus sp. T-3040, which had been isolated from soil. They were identified as cycloisomalto-heptaose, -octaose, and -nonaose, respectively, on the basis of their mass spectra, proton nuclear magnetic resonance spectra, carbon nuclear magnetic resonance spectra, infrared spectra, reducing power, and results of enzymatic analysis using endo-dextranase and exo-dextranase. They also showed features similar to cyclodextrins upon high-pressure liquid chromatography analysis using an ODS column.