Despite the economic importance of the carcinofauna which inhabits the estuarine regions of São Vicente, state of São Paulo, Brazil, there are few studies of the biology, distribution and biodiversity of crustaceans. The aim of this work was to list the species of macrocrustaceans that inhabit the non-consolidated substrates of Estuarine Bay Complex in São Vicente. After two years of monthly collections, we recorded two orders: Stomatopoda and Decapoda, represented by 13 families and 26 species. Only one species of the order Stomatopoda was recorded, all other 25 species belonging to the order Decapoda. Nine species of shrimp, in five families were captured; five of these belong to the family Penaeidae, one to Sergestidae one to Sycioniidae, one to Palaemonidae and one to Hippolytidae Anomuran crabs were composed of five species of hermit crabs. Brachyuran crabs were more prominent, with 11 species, most in the family Portunidae.