4
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Optimization of extraction of loquat flowers polyphenolics and its antioxidant and anti-polyphenol oxidase properties

      research-article

      Read this article at

      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          ABSTRACT

          In this study, the conditions of extraction of loquat flowers polyphenolics were optimized through response surface methodology (RSM). Proper extraction conditions were: solid to liquid ratio 1 g per 50 mL and ethanol concentration 50% at 61°C for 9 min. Furthermore, the antioxidant and anti-polyphenol oxidase (PPO) activity of purified total polyphenolics (PTP) were investigated. PTP displayed strong antioxidant activity with IC 50 values of 126.3 ± 8.9, 162.4 ± 6.3 and 94.97 mg ascorbic acid equivalent/g dry weight (mg AAE/d.w.) for ABTS, DPPH, and FRAP assays. In addition, PTP has a substantial inhibitory activity on PPO ( IC 50 = 115 ± 9.2 μg/mL). From the kinetics analysis, it was proved to be a reversible and mixed-type inhibitor of PPO with K I and K IS values of 76.77 μg/mL and 227.86 μg/mL, respectively. Further, the molecular mechanism underlying the inhibition of PPO by PTP was investigated by molecular docking techniques. The results showed that PTP units could form interaction with the catalytic pocket of PPO through the interaction with amino acid residues in the enzyme active center. The antioxidant activities of PTP together with its effect on PPO activity provide a strong starting point for their practical usage in the food industry.

          Graphical Abstract

          Related collections

          Most cited references20

          • Record: found
          • Abstract: found
          • Article: found
          Is Open Access

          Resources and Biological Activities of Natural Polyphenols

          The oxidative stress imposed by reactive oxygen species (ROS) plays an important role in many chronic and degenerative diseases. As an important category of phytochemicals, phenolic compounds universally exist in plants, and have been considered to have high antioxidant ability and free radical scavenging capacity, with the mechanism of inhibiting the enzymes responsible for ROS production and reducing highly oxidized ROS. Therefore, phenolic compounds have attracted increasing attention as potential agents for preventing and treating many oxidative stress-related diseases, such as cardiovascular diseases, cancer, ageing, diabetes mellitus and neurodegenerative diseases. This review summarizes current knowledge of natural polyphenols, including resource, bioactivities, bioavailability and potential toxicity.
            Bookmark
            • Record: found
            • Abstract: found
            • Article: found
            Is Open Access

            Tea Polyphenols in Promotion of Human Health

            Tea is the most widely used beverage worldwide. Japanese and Chinese people have been drinking tea for centuries and in Asia, it is the most consumed beverage besides water. It is a rich source of pharmacologically active molecules which have been implicated to provide diverse health benefits. The three major forms of tea are green, black and oolong tea based on the degree of fermentation. The composition of tea differs with the species, season, leaves, climate, and horticultural practices. Polyphenols are the major active compounds present in teas. The catechins are the major polyphenolic compounds in green tea, which include epigallocatechin-3-gallate (EGCG), epigallocatechin, epicatechin-3-gallate and epicatechin, gallocatechins and gallocatechin gallate. EGCG is the predominant and most studied catechin in green tea. There are numerous evidences from cell culture and animal studies that tea polyphenols have beneficial effects against several pathological diseases including cancer, diabetes and cardiovascular diseases. The polyphenolic compounds present in black tea include theaflavins and thearubigins. In this review article, we will summarize recent studies documenting the role of tea polyphenols in the prevention of cancer, diabetes, cardiovascular and neurological diseases.
              Bookmark
              • Record: found
              • Abstract: not found
              • Article: not found

              Antioxidant capacities and total phenolic contents of 62 fruits

                Bookmark

                Author and article information

                Journal
                Bioengineered
                Bioengineered
                KBIE
                kbie20
                Bioengineered
                Taylor & Francis
                2165-5979
                2165-5987
                2020
                3 March 2020
                : 11
                : 1
                : 281-290
                Affiliations
                Agricultural Product Storage and Processing Laboratory, Suzhou Academy of Agricultural Sciences , Suzhou, China
                Author notes
                CONTACT Yu-Ning Wang wyn705@ 123456163.com Agricultural Product Storage and Processing Laboratory, Suzhou Academy of Agricultural Sciences , Suzhou 215155, China
                Author information
                http://orcid.org/0000-0001-8663-5258
                Article
                1735604
                10.1080/21655979.2020.1735604
                7161536
                32125230
                519b5b7c-8a87-4698-93a5-da60b8fd2f3f
                © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

                This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

                History
                : 8 January 2020
                : 16 February 2020
                : 17 February 2020
                Page count
                Figures: 5, References: 20, Pages: 10
                Funding
                Funded by: Subsidized Project of Suzhou Academy of Agricultural Sciences
                Award ID: KJ (18) 101
                This work was supported by Grants from Subsidized Project of Suzhou Academy of Agricultural Sciences (grant number KJ (18) 101), Agricultural Science and Technology Independent Innovation Funds of Jiangsu Province (grant number CX (17) 3029) and The Science and Technology Foundation of Suzhou (grant number SNG201909).
                Categories
                Research Paper

                Biomedical engineering
                response surface methodology,antioxidants,purified loquat flowers polyphenolics,polyphenol oxidase,molecular docking

                Comments

                Comment on this article