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      Identification of myelin-associated glycoprotein as a major myelin-derived inhibitor of neurite growth

      , , , , ,
      Neuron
      Elsevier BV

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          Abstract

          Contact-dependent axon growth inhibitory activity is present in CNS myelin, but the inhibitory proteins have not been fully characterized. We report here that at least two peaks of inhibitory activity can be separated by fractionating solubilized CNS myelin proteins by DEAE chromatography. A major peak of inhibitory activity corresponded to the elution profile of myelin-associated glycoprotein (MAG). Immunodepletion of MAG from these inhibitory fractions removed neurite growth inhibition, whereas recombinant MAG (ectodomain) was a potent inhibitor of neurite outgrowth. Immunodepletion of MAG from total extracts of CNS myelin restored neurite growth up to 63% of control levels. These results establish that MAG is a significant, and possibly the major, inhibitor in CNS myelin; this has broad implications for axonal regeneration in the injured mammalian CNS.

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          Author and article information

          Journal
          Neuron
          Neuron
          Elsevier BV
          08966273
          October 1994
          October 1994
          : 13
          : 4
          : 805-811
          Article
          10.1016/0896-6273(94)90247-X
          7524558
          5200635d-9075-4790-9035-6ccc0355ede3
          © 1994

          https://www.elsevier.com/tdm/userlicense/1.0/

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