+1 Recommend
1 collections
      • Record: found
      • Abstract: found
      • Article: found

      The Internal Redox Balance of the Cells Influences the Metabolism of Lipids of Dietary Origin by J774 Macrophages: Implications for Foam Cell Formation


      Read this article at

          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.


          The effects of chylomicron remnants on lipid accumulation in J774 macrophages, and the incorporation of radioactivity from remnant lipids radiolabelled with [<sup>3</sup>H]oleate into cellular lipids was investigated. After 24 h of incubation with chylomicron remnants, there was considerable accumulation of lipid within the cells as assessed by staining with oil red O, indicating that the particles induce the formation of foam cells. Fatty acids released from the radiolabelled remnant lipids after uptake were found to be incorporated into cellular triacylglycerol (52%), phospholipid (37%) and cholesteryl ester (8%), but at higher remnant concentrations, the proportion used for triacylglycerol formation increased (up to 73%). When the macrophages were shifted into a pro-oxidising or pro-reducing state by incubation (24 h) with CuSO<sub>4</sub> (2.5 µ M) or N-acetylcysteine (5 m M), respectively, the incorporation of [<sup>3</sup>H]oleate from remnant lipid into cellular triacylglycerol and phospholipid was increased by 20–30% in the more oxidised as compared with the more reduced cells. These findings indicate that exposure of J774 macrophages to chylomicron remnants leads to the accumulation of lipid within the cells, and that this process is enhanced by pro-oxidising conditions. We conclude, therefore, that both lipids of dietary origin and the redox balance within macrophages may have a significant role in the induction of foam cell formation.

          Related collections

          Most cited references 5

          • Record: found
          • Abstract: not found
          • Article: not found

          Low density lipoprotein oxidation and its pathobiological significance.

            • Record: found
            • Abstract: not found
            • Article: not found

            Superoxide and hydrogen peroxide in relation to mammalian cell proliferation

             Roy Burdon (1995)
              • Record: found
              • Abstract: not found
              • Article: not found

              Macrophage Glutathione Content and Glutathione Peroxidase Activity Are Inversely Related to Cell-Mediated Oxidation of LDL


                Author and article information

                J Vasc Res
                Journal of Vascular Research
                S. Karger AG
                August 2001
                11 July 2001
                : 38
                : 4
                : 350-360
                aIstituto Superiore di Sanità, Laboratory of Metabolism and Pathological Biochemistry, Rome, Italy; bDepartment of Veterinary Basic Sciences, The Royal Veterinary College, London, UK
                51066 J Vasc Res 2001;38:350–360
                © 2001 S. Karger AG, Basel

                Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

                Page count
                Figures: 6, Tables: 2, References: 47, Pages: 11
                Research Paper


                Comment on this article