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<h5 class="section-title" id="d313433e253">BACKGROUND</h5>
<p id="P1">Premature infants often require oxygen supplementation and, therefore,
are exposed
to oxidative stress. Following oxygen exposure, preterm infants frequently develop
chronic lung disease and have a significantly increased risk of asthma.
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<h5 class="section-title" id="d313433e258">OBJECTIVE</h5>
<p id="P2">We sought to identify the underlying mechanisms by which neonatal hyperoxia
promotes
asthma development.
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<h5 class="section-title" id="d313433e263">METHODS</h5>
<p id="P3">Mice were exposed to neonatal hyperoxia followed by a period room air recovery.
A
group of mice were also intranasally exposed to house dust mite antigen (HDM). Assessments
were performed at various time points for evaluation of airway hyperresponsiveness
(AHR), eosinophilia, mucus production, inflammatory gene expression, T helper (Th)
and group 2 innate lymphoid cell (ILC2) responses. Sera from term- and preterm-born
infants were also collected and the levels of IL-33 and type 2 cytokines were measured.
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<h5 class="section-title" id="d313433e268">RESULTS</h5>
<p id="P4">Neonatal hyperoxia induced asthma-like features including AHR, mucus hyperplasia,
airway eosinophilia, and type 2 pulmonary inflammation. In addition, neonatal hyperoxia
promoted allergic Th responses to HDM exposure. Elevated IL-33 levels and ILC2 responses
were observed in the lungs most likely due to oxidative stress caused by neonatal
hyperoxia. IL-33 receptor signaling and ILC2s were vital for the induction of asthma-like
features following neonatal hyperoxia. Serum IL-33 levels correlated significantly
with serum levels of IL-5 and IL-13, but not IL-4 in preterm infants.
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<h5 class="section-title" id="d313433e273">CONCLUSION</h5>
<p id="P5">These data demonstrate that an axis involving IL-33 and ILC2s is important
for the
development of asthma-like features following neonatal hyperoxia and suggest therapeutic
potential for targeting IL-33, ILC2s, and oxidative stress to prevent and/or treat
asthma development related to prematurity.
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