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      • Record: found
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      Techniques for Analysis of Plant Phenolic Compounds

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          Abstract

          Phenolic compounds are well-known phytochemicals found in all plants. They consist of simple phenols, benzoic and cinnamic acid, coumarins, tannins, lignins, lignans and flavonoids. Substantial developments in research focused on the extraction, identification and quantification of phenolic compounds as medicinal and/or dietary molecules have occurred over the last 25 years. Organic solvent extraction is the main method used to extract phenolics. Chemical procedures are used to detect the presence of total phenolics, while spectrophotometric and chromatographic techniques are utilized to identify and quantify individual phenolic compounds. This review addresses the application of different methodologies utilized in the analysis of phenolic compounds in plant-based products, including recent technical developments in the quantification of phenolics.

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          Chemical studies of anthocyanins: A review

          Araceli Castañeda-Ovando, Ma Pacheco-Hernández, Ma. Páez-Hernández … (2009)
          Article 0 comments Cited 445 times – based on 0 reviews     Review now
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            Golden rules and pitfalls in selecting optimum conditions for high-speed counter-current chromatography.

            Yoichiro Ito (2005)
            This paper aims to be an aid to those chemists who are interested in utilizing high-speed counter-current chromatography (HSCCC), which is free of irreversible adsorption and offers high resolution comparable to column chromatography. It explains the selection of HSCCC conditions step by step including the selection of two-phase solvent systems, determination of partition coefficient (K) of analytes, preparation of two-phase solvent system and sample solution, selection of elution mode, flow rate, rotation speed, and on-line monitoring of the eluate. The paper covers both standard HSCCC and pH-zone-refining CCC techniques. Technical terms (italic) unfamiliar to the beginner are comprehensively explained in Glossary. Various examples of two-phase solvent systems used in HSCCC are listed in Appendices A and B. The commercial sources of HSCCC and other CCC instruments are described in detail in the study edited by Berthod [A. Berthod (Ed.), Counter-current Chromatography, Elsevier, Amsterdam, 2003].
            Article 0 comments Cited 163 times – based on 0 reviews     Review now
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              Cancer chemoprevention and chemotherapy: dietary polyphenols and signalling pathways.

              Sonia Ramos (2008)
              Prevention of cancer through dietary intervention recently has received an increasing interest, and dietary polyphenols have become not only important potential chemopreventive, but also therapeutic, natural agents. Polyphenols have been reported to interfere at the initiation, promotion and progression of cancer. They might lead to the modulation of proteins in diverse pathways and require the integration of different signals for the final chemopreventive or therapeutic effect. Polyphenols have been demonstrated to act on multiple key elements in signal transduction pathways related to cellular proliferation, differentiation, apoptosis, inflammation, angiogenesis and metastasis; however, these molecular mechanisms of action are not completely characterized and many features remain to be elucidated. The aim of this review is to provide insights into the molecular basis of potential chemopreventive and therapeutic activities of dietary polyphenols with emphasis in their ability to control intracellular signalling cascades considered as relevant targets in a cancer preventive approach.
              Article 0 comments Cited 158 times – based on 0 reviews     Review now
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                Author and article information

                Journal
                Journal ID (nlm-ta): Molecules
                Journal ID (iso-abbrev): Molecules
                Journal ID (publisher-id): molecules
                Title: Molecules
                Publisher: MDPI
                ISSN (Electronic): 1420-3049
                Publication date (Electronic): 19 February 2013
                Publication date Collection: February 2013
                Volume: 18
                Issue: 2
                Pages: 2328-2375
                Affiliations
                Department of Plant and Food Sciences, University of Sydney, Sydney, NSW 2006, Australia; E-Mails: ali.khoddami@ 123456sydney.edu.au (A.K.); meredith.wilkes@ 123456sydney.edu.au (M.A.W.)
                Author notes
                [* ] Author to whom correspondence should be addressed; E-Mail: thomas.roberts@ 123456sydney.edu.au ; Tel.: +61-2-8627-1042; Fax: +61-2-8627-1099.
                Article
                Publisher ID: molecules-18-02328
                DOI: 10.3390/molecules18022328
                PMC ID: 6270361
                PubMed ID: 23429347
                SO-VID: 521c6dda-00a1-408a-a3c6-556da312a8aa
                Copyright © © 2013 by the authors; licensee MDPI, Basel, Switzerland.
                License:

                This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license ( http://creativecommons.org/licenses/by/3.0/).

                History
                Date received : 25 October 2012
                Date revision received : 10 January 2013
                Date accepted : 31 January 2013
                Categories
                Subject: Review

                Keywords: food analysis,phenolic compound,phenolic extraction technique,phenolic quantification method,hplc,gc
                Data availability:
                Keywords: food analysis, phenolic compound, phenolic extraction technique, phenolic quantification method, hplc, gc

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