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      Automated detection of bacterial growth on 96-well plates for high-throughput drug susceptibility testing of Mycobacterium tuberculosis

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          Most cited references 8

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          The NumPy array: a structure for efficient numerical computation

          In the Python world, NumPy arrays are the standard representation for numerical data. Here, we show how these arrays enable efficient implementation of numerical computations in a high-level language. Overall, three techniques are applied to improve performance: vectorizing calculations, avoiding copying data in memory, and minimizing operation counts. We first present the NumPy array structure, then show how to use it for efficient computation, and finally how to share array data with other libraries.
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            Transforming clinical microbiology with bacterial genome sequencing.

            Whole-genome sequencing of bacteria has recently emerged as a cost-effective and convenient approach for addressing many microbiological questions. Here, we review the current status of clinical microbiology and how it has already begun to be transformed by using next-generation sequencing. We focus on three essential tasks: identifying the species of an isolate, testing its properties, such as resistance to antibiotics and virulence, and monitoring the emergence and spread of bacterial pathogens. We predict that the application of next-generation sequencing will soon be sufficiently fast, accurate and cheap to be used in routine clinical microbiology practice, where it could replace many complex current techniques with a single, more efficient workflow.
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              Resazurin microtiter assay plate: simple and inexpensive method for detection of drug resistance in Mycobacterium tuberculosis.

              A method for detecting multidrug-resistant Mycobacterium tuberculosis by using a reduction of resazurin is described. Eighty clinical isolates were evaluated against isoniazid and rifampin; results at 7 days were compared with those of the proportion method. Specificity and sensitivity were excellent. The method is simple, inexpensive, and rapid and might be used with other antituberculosis drugs.

                Author and article information

                Microbiology Society
                December 01 2018
                December 01 2018
                : 164
                : 12
                : 1522-1530
                [1 ] 2​National Institute for Health Research (NIHR) Oxford Biomedical Research Centre, John Radcliffe Hospital, Headley Way, Oxford, OX3 9DU, UK
                [2 ] 1​Nuffield Department of Medicine, John Radcliffe Hospital, University of Oxford, Headley Way, Oxford, OX3 9DU, UK
                [3 ] 3​National Mycobacterial Reference Service, National Infection Service, Public Health Laboratory Birmingham, Heartlands Hospital, Bordesley Green, Birmingham, B9 5SS, UK
                [4 ] 4​Emerging Bacterial Pathogens Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, Milan, Italy
                [5 ] 5​SYNLAB Gauting and Institute of Microbiology and Laboratory Medicine, World Health OrganizationSupranational Reference Laboratory of Tuberculosis, Munich-Gauting, Germany
                [6 ] 6​NIHR Health Protection Research Unit in Healthcare Associated Infection, Antimicrobial Resistance at University of Oxford in partnership with Public Health England, Oxford, UK
                © 2018


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