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      Simultaneous dermal matrix and autologous split-thickness skin graft transplantation in a porcine wound model: A three-dimensional histological analysis of revascularization : Revascularization of dermal matrix

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          Most cited references31

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          High-resolution episcopic microscopy: a rapid technique for high detailed 3D analysis of gene activity in the context of tissue architecture and morphology.

          We describe a new methodology for rapid 2D and 3D computer analysis and visualisation of gene expression and gene product pattern in the context of anatomy and tissue architecture. It is based on episcopic imaging of embryos and tissue samples, as they are physically sectioned, thereby producing inherently aligned digital image series and volume data sets, which immediately permit the generation of 3D computer representations. The technique uses resin as embedding medium, eosin for unspecific tissue staining, and colour reactions (beta-galactosidase/Xgal or BCIP/NBT) for specific labelling of gene activity and mRNA pattern. We tested the potential of the method for producing high-resolution volume data sets of adult human and porcine tissue samples and of specifically and unspecifically stained mouse, chick, quail, frog, and zebrafish embryos. The quality of the episcopic images resembles the quality of digital images of true histological sections with respect to resolution and contrast. Specifically labelled structures can be extracted using simple thresholding algorithms. Thus, the method is capable of quickly and precisely detecting molecular signals simultaneously with anatomical details and tissue architecture. It has no tissue restrictions and can be applied for analysis of human tissue samples as well as for analysis of all developmental stages of embryos of a wide variety of biomedically relevant species.
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            Biological background of dermal substitutes.

            Dermal substitutes are of major importance in treating full thickness skin defects, both in acute and chronic wounds. In this review we will outline specific requirements of three classes of dermal substitutes: Biological and clinical requirements will be translated to composition, physical structure, immunological properties and cell-matrix interactions of the various materials. Important properties like pore size, cell adhesion sites (e.g. RGD sequences), crosslinking, degradability and the presence of a basement membrane will be discussed for each of the different classes of materials. Copyright 2009 Elsevier Ltd and ISBI. All rights reserved.
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              Comparison of five dermal substitutes in full-thickness skin wound healing in a porcine model.

              The wound healing attributes of five acellular dermal skin substitutes were compared, in a two-step procedure, in a porcine model. Ten pigs were included in this experimental and randomized study. During the first step, dermal substitutes (Integra(®), ProDerm(®), Renoskin(®), Matriderm(®) 2mm and Hyalomatrix(®) PA) were implanted into full-thickness skin wounds and the epidermis was reconstructed during a second step procedure at day 21 using autologous split-thickness skin graft or cultured epithelial autograft. Seven pigs were followed-up for 2 months and 3 pigs for 6 months. Dermal substitute incorporation, epidermal graft takes, wound contraction and Vancouver scale were assessed, and histological study of the wounds was performed. Results showed significant differences between groups in dermis incorporation and in early wound contraction, but there was no difference in wound contraction and in Vancouver scale after 2 and 6 months of healing. We conclude there was no long-term difference of scar qualities in our study between the different artificial dermis. More, there was no difference between artificial dermis and the control group. This study makes us ask questions about the benefit of artificial dermis used in a two-step procedure. Copyright © 2012 Elsevier Ltd and ISBI. All rights reserved.
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                Author and article information

                Journal
                Wound Repair and Regeneration
                Wound Repair Regen
                Wiley
                10671927
                November 2014
                November 2014
                January 08 2015
                : 22
                : 6
                : 749-754
                Affiliations
                [1 ]Research Unit for Tissue Regeneration, Repair, and Reconstruction; Division of Plastic, Aesthetic, and Reconstructive Surgery; Department of Surgery; Medical University of Graz; Graz Austria
                [2 ]Center for Anatomy and Cell Biology; Medical University of Vienna; Vienna Austria
                [3 ]Institute of Pathology; Medical University of Graz; Graz Austria
                [4 ]Division of Biomedical Research; Medical University of Graz; Graz Austria
                Article
                10.1111/wrr.12233
                25358670
                524ddc12-5104-4e75-9d31-51576d0a97c6
                © 2015

                http://doi.wiley.com/10.1002/tdm_license_1.1

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