Point-wise and whole-field laser speckle intensity fluctuation measurements applied to botanical specimens

A new method is proposed to visualize the microcirculation map using a dynamic laser speckle effect. A skin surface is illuminated by He-Ne laser line spot and its image speckle is detected by a CCD array sensor. The erence between a pair of output data for successive scannings of the image speckles at the sensor plane was calculated and integrated for each pixel. The results were displayed in color graphics showing the spatial variation of the flow level in the area of interest.