The Oxidoreductase DsbA1 negatively influences 2,4-diacetylphloroglucinol biosynthesis by interfering the function of Gcd in  Pseudomonas fluorescens  2P24

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Abstract

Background

The polyketide antibiotic 2,4-diacetylphloroglucinol (2,4-DAPG), produced by Pseudomonas fluorescens 2P24, is positively regulated by the GacS-GacA two-component system.

Results

Here we reported on the characterization of DsbA1 (disulfide oxidoreductase) as novel regulator of biocontrol activity in P. fluorescens. Our data showed that mutation of dsbA1 caused the accumulation of 2,4-DAPG in a GacA-independent manner. Further analysis indicated that DsbA1 interacts with membrane-bound glucose dehydrogenase Gcd, which positively regulates the production of 2,4-DAPG. Mutation of cysteine (C)-235, C275, and C578 of Gcd, significantly reduced the interaction with DsbA1, enhanced the activity of Gcd and increased 2,4-DAPG production.

Conclusions

Our results suggest that DsbA1 regulates the 2,4-DAPG concentration via fine-tuning the function of Gcd in P. fluorescens 2P24.

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Most cited references 28

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Suppression of Root Diseases byPseudomonas fluorescensCHA0: Importance of the Bacterial Secondary Metabolite 2,4-Diacetylphloroglucinol

Christoph Keel (1992)

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Regulatory roles of the GacS/GacA two-component system in plant-associated and other gram-negative bacteria.

S Heeb, Andreas Haas (2001)

The sensor kinase GacS and the response regulator GacA are members of a two-component system that is present in a wide variety of gram-negative bacteria and has been studied mainly in enteric bacteria and fluorescent pseudomonads. The GacS/GacA system controls the production of secondary metabolites and extracellular enzymes involved in pathogenicity to plants and animals, biocontrol of soilborne plant diseases, ecological fitness, or tolerance to stress. A current model proposes that GacS senses a still-unknown signal and activates, via a phosphorelay mechanism, the GacA transcription regulator, which in turn triggers the expression of target genes. The GacS protein belongs to the unorthodox sensor kinases, characterized by an autophosphorylation, a receiver, and an output domain. The periplasmic loop domain of GacS is poorly conserved in diverse bacteria. Thus, a common signal interacting with this domain would be unexpected. Based on a comparison with the transcriptional regulator NarL, a secondary structure can be predicted for the GacA sensor kinases. Certain genes whose expression is regulated by the GacS/GacA system are regulated in parallel by the small RNA binding protein RsmA (CsrA) at a posttranscriptional level. It is suggested that the GacS/GacA system operates a switch between primary and secondary metabolism, with a major involvement of posttranscriptional control mechanisms.

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Agrobacterium tumefaciens DNA and PS8 bacteriophage DNA not detected in crown gall tumors.

T Currier, D Chilton, Stephen K. Farrand … (1974)

Renaturation kinetics of labeled Agrobacterium tumefaciens DNA are not influenced by addition of 10(4)-fold excess of crown gall tumor DNA. Reconstruction experiments demonstrated that 0.01% added bacterial DNA produces a detectable increase in rate of renaturation of labeled DNA. Crown gall tumor DNA therefore cannot contain as much as 0.01% A. tumefaciens DNA (one entire bacterial genome per three diploid tumor cells). By the same technique, PS8 bacteriophage DNA is not detected in crown gall tumor DNA under conditions that allow detection of 0.0007-0.001% added phage DNA. Crown gall tumor DNA cannot contain as much as one entire phage genome per diploid tumor cell. The presence of a small fraction of either genome (less than 5%), even if present as multiple copies, would escape detection by this method.

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Author and article information

Contributors

Xiaogang Wu: 4146308505@outlook.com

Li-Qun Zhang: zhanglq@cau.edu.cn

Journal

Journal ID (nlm-ta): BMC Microbiol

Journal ID (iso-abbrev): BMC Microbiol

Title: BMC Microbiology

Publisher: BioMed Central (London )

ISSN (Electronic): 1471-2180

Publication date (Electronic): 24 February 2020

Publication date PMC-release: 24 February 2020

Publication date Collection: 2020

Volume: 20

Electronic Location Identifier: 39

Affiliations

[1 ]GRID grid.256609.e, ISNI 0000 0001 2254 5798, College of Agriculture, , Guangxi University, ; Nanning, 530004 China

[2 ]GRID grid.22935.3f, ISNI 0000 0004 0530 8290, College of Plant Protection, , China Agricultural University, ; Beijing, 100193 China

Article

Publisher ID: 1714

DOI: 10.1186/s12866-020-1714-1

PMC ID: 7041245

PubMed ID: 32093646

SO-VID: 527cef02-fb6b-4689-a0b6-4505fbda7276

License:

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License ( http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

History

Date received : 16 September 2019

Date accepted : 27 January 2020

Funding

Funded by: FundRef http://dx.doi.org/10.13039/501100001809, National Natural Science Foundation of China;

Award ID: 31760533

Award ID: 31872020

Award Recipient : Xiaogang Wu Li-Qun Zhang

Funded by: the National Key Research and Development Program of China

Award ID: 2017YFD02011083

Award Recipient : Xiaogang Wu

Funded by: National Major Science and Technology Projects of China (CN)

Award ID: AA17204041

Award Recipient : Xiaogang Wu

Funded by: the Natural Science Foundation of Guangxi

Award ID: 2017GXNSFAA198341

Award Recipient : Xiaogang Wu

Custom metadata

ScienceOpen disciplines: Microbiology & Virology

Keywords: pseudomonas fluorescens,2,4-dapg,disulfide bond,oxidoreductase dsba1,glucose dehydrogenase gcd

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ScienceOpen disciplines: Microbiology & Virology

Keywords: pseudomonas fluorescens, 2,4-dapg, disulfide bond, oxidoreductase dsba1, glucose dehydrogenase gcd

The Oxidoreductase DsbA1 negatively influences 2,4-diacetylphloroglucinol biosynthesis by interfering the function of Gcd in Pseudomonas fluorescens 2P24

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Abstract

Background

Results

Conclusions

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Microbiology Society

Most cited references 28

Suppression of Root Diseases byPseudomonas fluorescensCHA0: Importance of the Bacterial Secondary Metabolite 2,4-Diacetylphloroglucinol

Regulatory roles of the GacS/GacA two-component system in plant-associated and other gram-negative bacteria.

Agrobacterium tumefaciens DNA and PS8 bacteriophage DNA not detected in crown gall tumors.

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