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      Quantitative and differential analysis of ciliate protozoa in rumen content samples filtered before and after fixation

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          Abstract

          The objective of this study was to assess whether the straining of rumen content samples influences the estimation of protozoal density. Ninety rumen samples were obtained from 30 cattle (three samples per animal). The samples were subjected to one of three treatments at the moment of collection: 1) fixation informalin without straining(control treatment), 2) straining before fixation informalin, or 3)straining after fixation informalin.To test the hypothesis of the variation in the protozoa composition in the samples, multivariate analyses with non-metric multidimensional scaling (NMDS) were carried out. The diversity and density of rumen protozoa were negatively affected by straining before fixation.In the pre-filtered sample, the number of ciliates from the genus Entodinium was reduced, and no individuals from the Diploplastron, Elytroplastron and Eudiplodinium genera were detected; these effects were not observed in the other two treatments. Straining after fixation did not interfere with the diversity of the ruminal community, but the abundance of protozoa was greater thanin the control treatment and significantly greater than in the samples filtered before fixation. These factors suggest that post-fixation straining is the recommended technique to analyze rumen protozoa.

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          Most cited references 13

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          Evaluation of sampling and fixation procedures used for counting rumen protozoa

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            Methods for the isolation, culture and assessment of the status of anaerobic rumen chytrids in both in vitro and in vivo systems.

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              Evaluation of a stratified continuous rumen incubation system

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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Journal
                rbz
                Revista Brasileira de Zootecnia
                R. Bras. Zootec.
                Sociedade Brasileira de Zootecnia (Viçosa )
                1806-9290
                November 2013
                : 42
                : 11
                : 831-834
                Affiliations
                [1 ] Universidade Federal de Juiz de Fora Brazil
                Article
                S1516-35982013001100010
                10.1590/S1516-35982013001100010
                Product
                Product Information: website
                Categories
                AGRICULTURE, DAIRY & ANIMAL SCIENCE
                VETERINARY SCIENCES

                Animal agriculture, General veterinary medicine

                Ciliophora, Entodinium, ruminal protozoa, ruminant

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