For Publishers
DiscoveryMetadataPeer reviewHostingPublishing
For Researchers
JoinPublishReviewCollect
Register
Blog
About
Search
Advanced search
My ScienceOpen
Search
For Publishers
For Researchers
Blog
About
30
views
20
references
 Top references
cited by
0
    
0 reviews
 Review
0
comments
 Comment
0
recommends
+1 Recommend
1 collections
    0
    shares
      497
      similar
       All similar
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Antimicrobial and cytotoxic activity of Alnus glutinosa (L.) Gaertn., A. incana (L.) Moench, and A. viridis (Chaix) DC. extracts

      research-article

      Read this article at

      ScienceOpenPublisher
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Introduction:

          The objective of the present study was to evaluate the antimicrobial and cytotoxic activities of water extracts of leaves and barks from Alnus glutinosa (L.) Gaertn., A. incana (L.) Moench, and A. viridis (Chaix) DC.

          Methods:

          The antimicrobial activities of extracts were tested against gram-negative and gram-positive bacteria as well as yeast strains by the agar diffusion method. The cell viability was determined by the Trypan blue dye exclusion method.

          Results:

          The largest diameters of inhibition zone (DIZ) were recorded with Staphylococcus aureus ATCC 6538 and Bacillus subtilis 168M. The highest percentage of cell viability was observed with water bark extracts of A. glutinosa (97.46%).

          Conclusions:

          Potential antimicrobial properties of A. glutinosa, A. incana, and A. viridis demonstrated in this study, as well as their low levels of toxicity, make them an interesting subject for further studies.

          Related collections

          Most cited references20

          • Record: found
          • Abstract: not found
          • Article: not found

          Culture of animal cells: a manual of basic techniques

          RL Freshney, I. FRESHNEY, I Freshney … (2000)
          Article 0 comments Cited 51 times – based on 0 reviews     Review now
            Bookmark
            • Record: found
            • Abstract: found
            • Article: found
            Is Open Access

            Screening of crude extracts of six medicinal plants used in South-West Nigerian unorthodox medicine for anti-methicillin resistant Staphylococcus aureus activity

            Kabir O Akinyemi, Olukayode Oladapo, Chidi Okwara … (2005)
            Background Six Nigerian medicinal plants Terminalia avicennioides, Phylantus discoideus, Bridella ferruginea, Ageratum conyzoides, Ocimum gratissimum and Acalypha wilkesiana used by traditional medical practitioners for the treatment of several ailments of microbial and non-microbial origins were investigated for in vitro anti-methicillin Resistant Staphylococcus aureus (MRSA) activity. Methods Fresh plant materials were collected from the users. Water and ethanol extracts of the shredded plants were obtained by standard methods. The Bacterial cultures used were strains of MRSA isolated from patients. MRSA was determined by the reference broth microdilution methods using the established National Committee for Clinical Laboratory Standards break points. Staphylococcus aureus NCIB 8588 was used as a standard strain. Susceptibility testing and phytochemical screening of the plant extracts were performed by standard procedures. Controls were maintained for each test batch. Results Both water and ethanol extracts of T. avicennioides, P. discoideus, O. gratissimum, and A. wilkesiana were effective on MRSA. The Minimum Inhibition Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the ethanol extracts of these plants range from 18.2 to 24.0 mcg/ml and 30.4 to 37.0 mcg/ml respectively. In contrast, MIC range of 30.6 to 43.0 mcg/ml and 55.4 to 71.0 mcg/ml were recorded for ethanol and water extracts of B. ferruginea, and A. conyzoides respectively. Higher MBC values were obtained for the two plants. These concentrations were too high to be considered active in this study. All the four active plants contained at least trace amount of anthraquinones. Conclusion Our results offer a scientific basis for the traditional use of water and ethanol extracts of A. wilkesiana, O. gratissimum, T. avicennioides and P. discoideus against MRSA-associated diseases. However, B. ferruginea and A. conyzoides were ineffective in vitro in this study; we therefore suggest the immediate stoppage of their traditional use against MRSA-associated diseases in Lagos, Nigeria.
            Article 0 comments Cited 45 times – based on 0 reviews     Review now
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Antibacterial, anti-inflammatory, anti-cholinesterase and mutagenic effects of extracts obtained from some trees used in South African traditional medicine.

              I Eldeen, Johan van Griensven, Esam Elgorashi (2005)
              Extracts obtained from 10 trees used in South African traditional medicine were screened for antibacterial, anti-inflammatory (COX-1 and COX-2) and anti-cholinesterase activities and investigated for potential mutagenic effects using the Ames test. Antibacterial activity was detected using the disc-diffusion and micro-dilution assays. The extracts were tested against Gram-positive bacteria: Bacillus subtilis, Staphylococcus aureus, Micrococcus luteus and Gram-negative bacteria: Escherichia coli and Klebsiella pneumoniae. Of the 78 different plant extracts investigated, 80% showed activity against both Gram-positive and Gram-negative bacteria in the disc-diffusion assay. In the micro-dilution assay, 60% of the plant extracts showed minimum inhibitory concentration (MIC) values 50% and 70% for water and organic solvent extracts, respectively). An ethyl acetate leaf extract of Trichilia dregeana showed selective inhibition of COX-2 (81%). In the acetylcholinesterase inhibitory test, 21% of the plant extracts were active at a concentration < or =1 mg ml(-1) using the micro-dilution assay. The lowest IC(50) value was 0.04 mg ml(-1) obtained with an ethanol bark extract of Combretum kraussii. None of the investigated plants showed any potential mutagenic effects.
              Article 0 comments Cited 35 times – based on 0 reviews     Review now
                Bookmark
                All references

                Author and article information

                Journal
                Journal ID (publisher-id): JHS
                Title: Journal of Health Sciences
                Publisher: University of Sarajevo Faculty of Health Studies (Bosnia )
                ISSN (Print): 2232-7576
                ISSN (Electronic): 1986-8049
                Publication date (Print): 2016
                Volume: 6
                Issue: 2
                Pages: 100-104
                Affiliations
                [1 ]Department of Biology, Faculty of Science, University of Sarajevo, Sarajevo, Bosnia and Herzegovina
                [2 ]Institute for Genetic Engineering and Biotechnology, Sarajevo, Bosnia and Herzegovina
                Author notes
                [* ]Corresponding author: Sabina Dahija, Department of Biology, Faculty of Science, University of Sarajevo, Zmaja od Bosne 33-35, 71000 Sarajevo, Bosnia and Herzegovina, Tel.: +387 33 723 702. E-mail: sabina_dudevic@ 123456yahoo.com
                Article
                Publisher ID: JHS-6-100
                DOI: 10.17532/jhsci.2016.328
                SO-VID: 52c73949-1c70-4a51-97d0-b1ea05b717d6
                Copyright © Copyright: © 2016 Sabina Dahija et al.
                License:

                This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

                History
                Date received : 04 February 2016
                Date accepted : 04 June 2016
                Categories
                Subject: RESEARCH ARTICLE

                ScienceOpen disciplines: Nursing,General medicine,Medicine,Molecular medicine,Life sciences
                Keywords: Antibacterial activity,antifungal activity,cytotoxic activity,Alnus
                Data availability:
                ScienceOpen disciplines: Nursing, General medicine, Medicine, Molecular medicine, Life sciences
                Keywords: Antibacterial activity, antifungal activity, cytotoxic activity, Alnus

                Comments

                Comment on this article