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      Ultrastructural Study of the Cytoskeleton of Optic Nerve Axons in Guinea Pigs as Revealed by a Quick-Freezing, Deep-Etching Method


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          The ultrastructure of the cytoskeleton of optic nerve axons in guinea pigs was examined by the quick-freezing and deep-etching (QF-DE) method. The optic nerve tissues were treated with 0.5% saponin before QF. In the replicas, the axoplasm, as observed with conventional ultrathin sections, was seen to be composed of longitudinally oriented microtubules (MT) and neurofilaments (NF). Thin and elaborate cross-linking structures were observed in the interstices between MTs, NFs, and membranous organelles. They consisted of two different types: NF-associated cross-linking structures (about 20-50 nm in length) and MT-associated structures (about 10-20 nm in length), and may play a role in the slow transport of NF and MT and fast transport of organelles, respectively.

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          Author and article information

          Ophthalmic Res
          Ophthalmic Research
          S. Karger AG
          11 December 2009
          : 28
          : 1
          : 29-35
          Departments of aOphthalmology and bAnatomy, Yamanashi Medical University, Tamaho, Yamanashi, Japan
          267870 Ophthalmic Res 1996;28:29–35
          © 1996 S. Karger AG, Basel

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          : 10 July 1995
          : 26 September 1995
          Page count
          Pages: 7
          Original Paper

          Vision sciences,Ophthalmology & Optometry,Pathology
          Optic nerve axon,Cross-linking structure,Guinea pig,Saponin,Quick-freezing, deep-etching method


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