The influence of laminar shear stress on cell proliferation was investigated for subconfluent
bovine aortic endothelial cell monolayers seeded on either glass or Thermanox. The
effect of both steady and pulsatile shear stress was studied. For bovine aortic endothelial
cells on Thermanox exposed to steady flow, shear stress levels greater than 15 dyne/cm2
resulted in a dose-related reduction in the rate of cell proliferation. At 90 dyne/cm2,
the rate of proliferation was virtually totally arrested for 48 h, but then resumed.
Pulsatile shear stress produced an exaggeration of the effect observed in response
to steady shear stress. Bovine aortic endothelial cells seeded on glass, exhibited
a similar but more sensitive response, with a significant reduction in growth rate
observed after 24 h at shear stress levels greater than 5 dyne/cm2 and a near cessation
of proliferation at 13 dyne/cm2.