26
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Tango1 spatially organizes ER exit sites to control ER export

      research-article

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Drosophila melanogaster Tango1 is required for secretion of Collagen IV. Liu et al. use a genetic analysis to show that Tango1 is required to spatially maintain the size and integrity of ER exit site–Golgi units and that loss of Tango1 function impairs not only Collagen IV secretion but also general secretion.

          Abstract

          Exit of secretory cargo from the endoplasmic reticulum (ER) takes place at specialized domains called ER exit sites (ERESs). In mammals, loss of TANGO1 and other MIA/cTAGE (melanoma inhibitory activity/cutaneous T cell lymphoma–associated antigen) family proteins prevents ER exit of large cargoes such as collagen. Here, we show that Drosophila melanogaster Tango1, the only MIA/cTAGE family member in fruit flies, is a critical organizer of the ERES–Golgi interface. Tango1 rings hold COPII (coat protein II) carriers and Golgi in close proximity at their center. Loss of Tango1, present at ERESs in all tissues, reduces ERES size and causes ERES–Golgi uncoupling, which impairs secretion of not only collagen, but also all other cargoes we examined. Further supporting an organizing role of Tango1, its overexpression creates more and larger ERESs. Our results suggest that spatial coordination of ERES, carrier, and Golgi elements through Tango1’s multiple interactions increases secretory capacity in Drosophila and allows secretion of large cargo.

          Related collections

          Most cited references60

          • Record: found
          • Abstract: found
          • Article: not found

          The mechanisms of vesicle budding and fusion.

          Genetic and biochemical analyses of the secretory pathway have produced a detailed picture of the molecular mechanisms involved in selective cargo transport between organelles. This transport occurs by means of vesicular intermediates that bud from a donor compartment and fuse with an acceptor compartment. Vesicle budding and cargo selection are mediated by protein coats, while vesicle targeting and fusion depend on a machinery that includes the SNARE proteins. Precise regulation of these two aspects of vesicular transport ensures efficient cargo transfer while preserving organelle identity.
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            Construction of transgenic Drosophila by using the site-specific integrase from phage phiC31.

            The phiC31 integrase functions efficiently in vitro and in Escherichia coli, yeast, and mammalian cells, mediating unidirectional site-specific recombination between its attB and attP recognition sites. Here we show that this site-specific integration system also functions efficiently in Drosophila melanogaster in cultured cells and in embryos. Intramolecular recombination in S2 cells on transfected plasmid DNA carrying the attB and attP recognition sites occurred at a frequency of 47%. In addition, several endogenous pseudo attP sites were identified in the fly genome that were recognized by the integrase and used as substrates for integration in S2 cells. Two lines of Drosophila were created by integrating an attP site into the genome with a P element. phiC31 integrase injected into embryos as mRNA functioned to promote integration of an attB-containing plasmid into the attP site, resulting in up to 55% of fertile adults producing transgenic offspring. A total of 100% of these progeny carried a precise integration event at the genomic attP site. These experiments demonstrate the potential for precise genetic engineering of the Drosophila genome with the phiC31 integrase system and will likely benefit research in Drosophila and other insects.
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Analysis of genetic mosaics in developing and adult Drosophila tissues.

              T Xu, G Rubin (1993)
              We have constructed a series of strains to facilitate the generation and analysis of clones of genetically distinct cells in developing and adult tissues of Drosophila. Each of these strains carries an FRT element, the target for the yeast FLP recombinase, near the base of a major chromosome arm, as well as a gratuitous cell-autonomous marker. Novel markers that carry epitope tags and that are localized to either the cell nucleus or cell membrane have been generated. As a demonstration of how these strains can be used to study a particular gene, we have analyzed the developmental role of the Drosophila EGF receptor homolog. Moreover, we have shown that these strains can be utilized to identify new mutations in mosaic animals in an efficient and unbiased way, thereby providing an unprecedented opportunity to perform systematic genetic screens for mutations affecting many biological processes.
                Bookmark

                Author and article information

                Journal
                J Cell Biol
                J. Cell Biol
                jcb
                jcb
                The Journal of Cell Biology
                The Rockefeller University Press
                0021-9525
                1540-8140
                03 April 2017
                : 216
                : 4
                : 1035-1049
                Affiliations
                [1]School of Life Sciences, Tsinghua University, Beijing 100084, China
                Author notes
                Correspondence to José Carlos Pastor-Pareja: jose.pastor@ 123456biomed.tsinghua.edu.cn
                [*]

                M. Liu and Z. Feng contributed equally to this paper.

                Author information
                http://orcid.org/0000-0001-7553-7425
                http://orcid.org/0000-0002-9740-0435
                http://orcid.org/0000-0002-9942-4158
                http://orcid.org/0000-0002-8487-915X
                http://orcid.org/0000-0002-5720-1466
                http://orcid.org/0000-0002-2032-7292
                http://orcid.org/0000-0003-3934-9309
                http://orcid.org/0000-0002-3823-4473
                Article
                201611088
                10.1083/jcb.201611088
                5379956
                28280122
                534371a9-e6e9-4d06-9f3e-191f666b467a
                © 2017 Liu et al.

                This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

                History
                : 16 November 2016
                : 10 January 2017
                : 11 January 2017
                Funding
                Funded by: National Science Foundation of China, DOI https://doi.org/10.13039/501100001809;
                Award ID: 31371459
                Award ID: 31550110204
                Funded by: Tsinghua University, DOI https://doi.org/10.13039/501100004147;
                Award ID: 20131089281
                Categories
                Research Articles
                Article
                34
                40
                38

                Cell biology
                Cell biology

                Comments

                Comment on this article