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      Mouse model of chronic and binge ethanol feeding (the NIAAA model).

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          Abstract

          Chronic alcohol consumption is a leading cause of chronic liver disease worldwide, leading to cirrhosis and hepatocellular carcinoma. Currently, the most widely used model for alcoholic liver injury is ad libitum feeding with the Lieber-DeCarli liquid diet containing ethanol for 4-6 weeks; however, this model, without the addition of a secondary insult, only induces mild steatosis, slight elevation of serum alanine transaminase (ALT) and little or no inflammation. Here we describe a simple mouse model of alcoholic liver injury by chronic ethanol feeding (10-d ad libitum oral feeding with the Lieber-DeCarli ethanol liquid diet) plus a single binge ethanol feeding. This protocol for chronic-plus-single-binge ethanol feeding synergistically induces liver injury, inflammation and fatty liver, which mimics acute-on-chronic alcoholic liver injury in patients. This feeding protocol can also be extended to chronic feeding for longer periods of time up to 8 weeks plus single or multiple binges. Chronic-binge ethanol feeding leads to high blood alcohol levels; thus, this simple model will be very useful for the study of alcoholic liver disease (ALD) and of other organs damaged by alcohol consumption.

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          Author and article information

          Journal
          Nat Protoc
          Nature protocols
          Springer Science and Business Media LLC
          1750-2799
          1750-2799
          Mar 2013
          : 8
          : 3
          Affiliations
          [1 ] Laboratory of Liver Diseases, National Institute on Alcohol Abuse and Alcoholism NIAAA, National Institutes of Health NIH, Bethesda, Maryland, USA.
          Article
          nprot.2013.032 NIHMS512523
          10.1038/nprot.2013.032
          3788579
          23449255
          534ebb1d-3a1b-4506-bf7e-e01e79d839c8
          History

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