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      Growth of an Ocular Strain of Chlamydia trachomatis on Chang Conjunctival Cells

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          Abstract

          The most widely used cell lines for in vitro studies of Chlamydia trachomatis infections are McCoy and HeLa cells. Our understanding of the intracellular events that occur during ocular C. trachomatis infections may well be improved if such infections could be studied in a host cell that more closely approximates the natural host conjunctival cell. We report here that Chang conjunctival cells, which have been derived from human conjunctival cells, can be infected with C. trachomatis, serovar B. The optimal times for exposure of the host cells to infectious material and to cycloheximide are about 24 and 48 h, respectively. Under these conditions, the maximum number of inclusions is observed at 72 h after centrifugation.

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          Author and article information

          Journal
          ORE
          Ophthalmic Res
          10.1159/issn.0030-3747
          Ophthalmic Research
          S. Karger AG
          0030-3747
          1423-0259
          1989
          1989
          10 December 2009
          : 21
          : 5
          : 388-391
          Affiliations
          Department of Biological Sciences, Mount Holyoke College, South Hadley, Mass., USA
          Article
          266907 Ophthalmic Res 1989;21:388–391
          10.1159/000266907
          2601944
          © 1989 S. Karger AG, Basel

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          Pages: 4
          Categories
          Original Paper

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