The Prognostic Value of Circulating Cell-Free DNA in Colorectal Cancer: A Meta-Analysis

There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

Abstract

Background: Circulating cell-free DNA (cfDNA) is a promising candidate biomarker for detection, monitoring and survival prediction of colorectal cancer (CRC). However, its prognostic significance for patients with CRC remains controversial. To derive a precise estimation of the prognostic significance of cfDNA, a meta-analysis was performed.

Methods: We made a systematic search in data base of the Science Citation Index Embase and Pubmed for studies reporting prognostic data of cfDNA in CRC patients. The data of cfDNA on recurrences-free survival (RFS) and overall survival (OS) were extracted and measured in hazard rates (HRs) and 95% confident intervals (CIs). Subgroup analyses were carried out as well. Finally, the meta-analysis is accompanied with nine studies including 19 subunits.

Results: The pooled HRs with 95% CIs revealed strong associations between cfDNA and RFS (HR [95%CI]=2.78[2.08-3.72], I ²=32.23%, n=7) along with OS (HR [95%CI]=3.03[2.51-3.66], I ²=29.24%, n=12) in patients with CRC. Entire subgroup analyses indicated strong prognostic value of cfDNA irrespective tumor stage, study size, tumor markers, detection methods and marker origin.

Conclusions: All the results exhibits that appearance of cfDNA in blood is an indicator for adverse RFS and OS in CRC patients.

Related collections

Most cited references 45

Record: found
Abstract: found
Article: not found

Extracting summary statistics to perform meta-analyses of the published literature for survival endpoints.

M Parmar, V. Torri, L. Stewart (1998)

Meta-analyses aim to provide a full and comprehensive summary of related studies which have addressed a similar question. When the studies involve time to event (survival-type) data the most appropriate statistics to use are the log hazard ratio and its variance. However, these are not always explicitly presented for each study. In this paper a number of methods of extracting estimates of these statistics in a variety of situations are presented. Use of these methods should improve the efficiency and reliability of meta-analyses of the published literature with survival-type endpoints.

0 comments Cited 834 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: not found

Approaches to heterogeneity in meta-analysis.

D Petitti (2001)

This paper reviews publications from January 1999 to March 2001 on reproductive health topics that were self-identified as meta-analysis or were indexed as meta-analysis in MEDLINE. It sought to assess whether tests of statistical heterogeneity were done, whether the results were reported, and how a finding of significance for a test of statistical heterogeneity was handled and the results interpreted. The review identified some concerns. Tests of statistical heterogeneity were not done universally even though virtually all writers on the topic emphasize their importance. Even when done, results of these tests were not universally reported. Although the consensus appears to be that heterogeneity tests are conservative for meta-analysis of studies and a probability value of 0.10 is preferred, many meta-analyses used the conventional value of 0.05 without providing a reason. The rationale for the choice of a random or fixed effects model was not generally evident. The review also provided some positive models and some recommendations for assessing, reporting and exploring heterogeneity are made considering these models and the published recommendations of experts. Copyright 2001 John Wiley & Sons, Ltd.

0 comments Cited 113 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: not found

Increased plasma DNA integrity in cancer patients.

Richard Roden, Michael R Bristow, Chien-Jui Cheng … (2003)

Tumor-released DNA in blood represents a promising biomarker for cancer detection. It has been postulated that tumor necrosis causes release of DNA of varying sizes, which contrasts apoptosis in normal tissue that releases smaller and more uniform DNA fragments. To test the hypothesis that increased DNA integrity, i.e., a longer DNA strand, is a tumor-associated marker in plasma, we determined the genomic DNA integrity index in plasma DNA using real-time PCR assays. A DNA integrity index and DNA concentration in plasma were determined in 61 patients with gynecological and breast cancers and 65 female patients without neoplastic diseases. We found that the area under the receiver-operating characteristic curve for DNA integrity index was 0.911 for cancer versus nonneoplastic patients. Given 100% specificity, the highest sensitivity achieved in detecting the cancer group was 62% (95% confidence interval = 0.50-0.74) at the index cutoff of 0.59. Fifty percent of stage I cancers had a DNA integrity index above this cutoff. All 11 patients with benign adnexal masses that clinically can be confused with malignant gynecological neoplasms demonstrated DNA integrity index < 0.59. Our findings suggest that increased DNA integrity in plasma DNA is associated with cancer, and measurement of DNA integrity may provide a simple and inexpensive measure for cancer detection.

0 comments Cited 97 times – based on 0 reviews      Review now

Bookmark

All references

Author and article information

Journal

Journal ID (nlm-ta): J Cancer

Journal ID (iso-abbrev): J Cancer

Journal ID (publisher-id): jca

Title: Journal of Cancer

Publisher: Ivyspring International Publisher (Sydney )

ISSN (Electronic): 1837-9664

Publication date Collection: 2016

Publication date (Electronic): 4 June 2016

Volume: 7

Issue: 9

Pages: 1105-1113

Affiliations

1. Department of Gastrointestinal Surgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China;

2. Department of Research Administration, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.

Author notes

✉ Corresponding authors: Hai-yan Ge, Email: ghy1333@ 123456easthospital.cn Zhen-Yu Zhang Email : zhangsurgery@ 123456qq.com . Department of Gastrointestinal Surgery, Shanghai East Hospital, Tongji University School of Medicine, Pudong New District, No. 150, Jimo Road, Shanghai, 200120, China. Tel: +86 21 38804518 Fax: +86 21 58798999.

Competing Interests: The authors declare that they have no potential competing interests.

Article

Publisher ID: jcav07p1105

DOI: 10.7150/jca.14801

PMC ID: 4911878

PubMed ID: 27326254

SO-VID: 53c9007f-bc54-4e56-ab67-851e07b87d7e

Copyright © © Ivyspring International Publisher. Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited. See http://ivyspring.com/terms for terms and conditions.

The Prognostic Value of Circulating Cell-Free DNA in Colorectal Cancer: A Meta-Analysis

Read this article at

Abstract

Related collections

Karger: Oncology

Most cited references 45

Extracting summary statistics to perform meta-analyses of the published literature for survival endpoints.

Approaches to heterogeneity in meta-analysis.

Increased plasma DNA integrity in cancer patients.

Author and article information

Journal

Affiliations

Author notes

Article

History

Categories

Comments

Comment on this article

Similar content 511

Cited by 29

Most referenced authors 1,215