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      Staphylococcus aureus and Staphylococcus epidermidis strain diversity underlying pediatric atopic dermatitis

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          Abstract

          <p class="first" id="P1">The heterogeneous course, severity, and treatment responses among patients with atopic dermatitis (AD; eczema) highlight the complexity of this multifactorial disease. Prior studies have used traditional typing methods on cultivated isolates or sequenced a bacterial marker gene to study the skin microbial communities of AD patients. Shotgun metagenomic sequence analysis provides much greater resolution, elucidating multiple levels of microbial community assembly ranging from kingdom to species and strain-level diversification. Here, we analyze microbial temporal dynamics from a cohort of pediatric AD patients sampled throughout the disease course. Species-level investigation of AD flares showed greater <i>Staphylococcus aureus</i>-predominance in patients with more severe disease and <i>S. epidermidis</i>-predominance in patients with less severe disease. At the strain-level, metagenomic sequencing analyses demonstrated clonal <i>S. aureus</i> strains in more severe patients and heterogeneous <i>S. epidermidis</i> strain communities in all patients. To investigate strain-level biological effects of <i>S. aureus</i>, we topically colonized mice with strains isolated from AD patients and controls. This cutaneous colonization model demonstrated <i>S. aureus</i> strain-specific differences in eliciting skin inflammation and immune signatures characteristic of AD patients. Specifically, <i>S. aureus</i> isolates from AD patients with more severe flares induced epidermal thickening and expansion of cutaneous Th2 and Th17 cells. Integrating high-resolution sequencing, culturing, and animal models demonstrated how functional differences of staphylococcal strains may contribute to the complexity of AD disease. </p>

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          Author and article information

          Journal
          Science Translational Medicine
          Sci. Transl. Med.
          American Association for the Advancement of Science (AAAS)
          1946-6234
          1946-6242
          July 05 2017
          July 05 2017
          : 9
          : 397
          : eaal4651
          Affiliations
          [1 ]NISC Comparative Sequencing Program
          Article
          10.1126/scitranslmed.aal4651
          5706545
          28679656
          53e52ff7-8533-4967-b1ef-20dcca36f385
          © 2017

          http://www.sciencemag.org/about/science-licenses-journal-article-reuse

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