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Comparative Study of the Sarcoplasmic Proteins of Vascular and Some Other Cow Smooth and Striated Muscles by Starch-Gel Electrophoresis
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Abstract
To extend the application of starch-gel electrophoresis to the sarcoplasmic proteins of vertebrate smooth muscle, these extracts must be fractionated by acidification at pH 5 and by ammonium sulphate at 45 % saturation in order to remove the structural proteins and some contaminants which impede the resolution. The extracts of six cow muscles – a white skeletal one, a red skeletal one, the cardiac, stomach, uterus, and carotid muscles – have been analyzed by this method. The patterns have been stained for the major glycolytic enzymes. Enolase, creatine kinase, and lactate dehydrogenase (LDH) make discrimination of the smooth and the striated muscles possible. The smooth muscles contain a much lower amount of glycolytic enzymes and a large number of nonglycolytic bands. Their LDH isoenzymic distribution confirms their high aerobicity. Although many bands are common to the three smooth muscles examined, starch-gel electrophoresis is also able to detect protein differences between the smooth vertebrate muscles of the same species.
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157958 Angiologica 1973;10:31–49Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.